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Literature summary extracted from

  • Chang, T.Y.; Prescott, L.M.; Jones, M.E.
    Aspartate carbamyltransferase (Streptococcus faecalis) (1978), Methods Enzymol., 51, 41-50.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.3.2 4-aminobutyrate 400% activation Enterococcus faecalis
2.1.3.2 beta-Alanine 600% activation Enterococcus faecalis
2.1.3.2 Butyrate 5700% activation Enterococcus faecalis
2.1.3.2 Carbamoyl-beta-alanine 5000% activation Enterococcus faecalis
2.1.3.2 D-Aspartate 5700% activation Enterococcus faecalis
2.1.3.2 formate activates Enterococcus faecalis
2.1.3.2 Isobutyrate 5800% activation Enterococcus faecalis
2.1.3.2 L-2-aminobutyrate 1400% activation Enterococcus faecalis
2.1.3.2 L-alanine 800% activation Enterococcus faecalis
2.1.3.2 additional information every type of anion tested, including ATP and CTP can stimulate the reaction to a certain extent Enterococcus faecalis
2.1.3.2 propionate 5600% activation Enterococcus faecalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.2 HgCl2 0.001 mM, 50% inhibition Enterococcus faecalis
2.1.3.2 Mersalyl 0.01 mM, 50% inhibition Enterococcus faecalis
2.1.3.2 p-mercuribenzoate 0.01 mM, 50% inhibition Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.2 0.2
-
 Carbamoyl phosphate at high salt concentrations Enterococcus faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.2 32500
-
 4 * 32500, SDS-PAGE Enterococcus faecalis
2.1.3.2 125000
-
 sucrose density gradient centrifugation Enterococcus faecalis
2.1.3.2 128000
-
 sedimentation equilibrium Enterococcus faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.2 carbamoylphosphate + L-aspartate Enterococcus faecalis
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.2 Enterococcus faecalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.2 pH 4.8, hydroxylapatite, ammonium sulfate, DEAE-Sephadex Enterococcus faecalis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Enterococcus faecalis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.2 350
-
-
 Enterococcus faecalis

Storage Stability

EC Number Storage Stability Organism
2.1.3.2 -20°C, 0.1-1 mg protein/ml, at least 2 years, no loss of activity 4°C, 0.1-1 mg/ml, at least 2 months, no loss of activity Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Enterococcus faecalis phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Enterococcus faecalis phosphate + N-carbamoyl-L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.2 tetramer 4 * 32500, SDS-PAGE Enterococcus faecalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.3.2 6.6
-
 stable Enterococcus faecalis