EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.2.8 | structural gene 42 encodes enzyme | Tequatrovirus T4 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.2.8 | C148G | mutant is unable to catalyze a detectable 18O exchange in either the absence or presence of the folate cofactor | Tequatrovirus T4 |
2.1.2.8 | D179N | mutant with altered substrate preference, favoring dUMP rather than dCMP | Tequatrovirus T4 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.8 | 10-methyl-5,8-dideazatetrahydrofolate | competitive inhibitor with respect to tetrahydrofolate | Tequatrovirus T4 | |
2.1.2.8 | 5-deazatetrahydrofolate | competitive inhibitor with respect to tetrahydrofolate | Tequatrovirus T4 | |
2.1.2.8 | 5-fluorodeoxyuridylate | - |
Tequatrovirus T4 | |
2.1.2.8 | 5-hydroxymethyl-dCMP | competitor of dCMP, inhibits formation of 5-hydroxymethyl-dCMP | Tequatrovirus T4 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.8 | 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | Tequatrovirus T4 | - |
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | 5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4 | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.8 | Tequatrovirus T4 | - |
Escherichia coli infected with bacteriophage T4 | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.1.2.8 | 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate = tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | catalytic mechanism | Tequatrovirus T4 |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.2.8 | 3.1 | - |
dCMP formation | Tequatrovirus T4 |
2.1.2.8 | 4.6 | - |
5-hydroxymethyl-dCMP formation | Tequatrovirus T4 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.8 | 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | - |
Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | 5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4 | ? | |
2.1.2.8 | 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | Cys-148 is catalytically essential | Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | - |
r | |
2.1.2.8 | additional information | enzyme also catalyzes 18O exchange from solvent water into 5-hydroxymethyl-dCMP in the absence of exogenous tetrahydrofolate and in the presence of tetrahydrofolate analogues that lack N-5, mechanism | Tequatrovirus T4 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.2.8 | homodimer | - |
Tequatrovirus T4 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.8 | 5,10-methylenetetrahydrofolate | - |
Tequatrovirus T4 | |
2.1.2.8 | tetrahydrofolate | - |
Tequatrovirus T4 |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.8 | 0.01 | - |
10-methyl-5,8-dideazatetrahydrofolate | - |
Tequatrovirus T4 | |
2.1.2.8 | 0.1 | - |
5-hydroxymethyl-dCMP | - |
Tequatrovirus T4 | |
2.1.2.8 | 0.11 | - |
5-deazatetrahydrofolate | - |
Tequatrovirus T4 |