Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Garcia-Fuentes, L.; Reche, P.; Lopez-Mayorga, O.; Santi, D.V.; Gonzalez-Pacanowska, D.; Baron, C.
    Thermodynamic analysis of the binding of 5-fluoro-2'-deoxyuridine 5'-monophosphate to thymidylate synthase over a range of temperatures (1995), Eur. J. Biochem., 232, 641-645.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.45 medicine enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs Lacticaseibacillus casei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45 Escherichia coli chi2913 transformed with pKPTSd containing the complete coding sequence for Lactobacillus casei TS Lacticaseibacillus casei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.45
-
 Escherichia coli
2.1.1.45
-
 Lacticaseibacillus casei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 5-fluoro-2'-deoxyuridine 5'-monophosphate
-
 Lacticaseibacillus casei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 36000
-
 2 * 36000 Lacticaseibacillus casei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Lacticaseibacillus casei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45 recombinant enzyme Lacticaseibacillus casei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.45 2.5 3
-
 Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Escherichia coli dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Lacticaseibacillus casei dihydrofolate + dTMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.45 dimer
-
 Escherichia coli
2.1.1.45 dimer 2 * 36000 Lacticaseibacillus casei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.45 16 35
-
 Lacticaseibacillus casei

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.45 (+)-methylenetetrahydrofolate
-
 Escherichia coli
2.1.1.45 (+)-methylenetetrahydrofolate
-
 Lacticaseibacillus casei