BRENDA - Enzyme Database

Characterization of S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase involved in floral scent production in Clarkia breweri

Wang, J.; Pichersky, E.; Arch. Biochem. Biophys. 349, 153-160 (1998)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
2.1.1.146
expression in Escherichia coli
Clarkia breweri
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.146
Ca2+
5 mM, weak inhibition
Clarkia breweri
2.1.1.146
Cu2+
5 mM, strong inhibition
Clarkia breweri
2.1.1.146
Fe2+
5 mM, strong inhibition
Clarkia breweri
2.1.1.146
Fe3+
5 mM, strong inhibition
Clarkia breweri
2.1.1.146
K+
5 mM, weak inhibition
Clarkia breweri
2.1.1.146
Mg2+
5 mM, 33% inhibition
Clarkia breweri
2.1.1.146
Mn2+
5 mM, strong inhibition
Clarkia breweri
2.1.1.146
Na+
5 mM, weak inhibition
Clarkia breweri
2.1.1.146
NH4+
5 mM, weak inhibition
Clarkia breweri
2.1.1.146
Zn2+
5 mM, strong inhibition
Clarkia breweri
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.146
0.007
-
eugenol
plant-purified enzyme
Clarkia breweri
2.1.1.146
0.019
-
S-adenosyl-L-methionine
recombinant enzyme
Clarkia breweri
2.1.1.146
0.027
-
S-adenosyl-L-methionine
plant-purified enzyme
Clarkia breweri
2.1.1.146
0.03
-
eugenol
recombinant enzyme
Clarkia breweri
2.1.1.146
0.058
-
isoeugenol
plant-purified enzyme
Clarkia breweri
2.1.1.146
0.074
-
isoeugenol
recombinant enzyme
Clarkia breweri
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.1.1.146
additional information
methylation of eugenol and isoeugenol
Clarkia breweri
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.146
40000
-
2 * 40000, SDS-PAGE
Clarkia breweri
2.1.1.146
88000
-
gel filtration
Clarkia breweri
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.1.1.146
Clarkia breweri
O04385
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.1.1.146
-
Clarkia breweri
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.146
flower
-
Clarkia breweri
-
2.1.1.146
petal
-
Clarkia breweri
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.1.1.146
additional information
-
-
Clarkia breweri
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.1.1.146
S-adenosyl-L-methionine + eugenol
-
485153
Clarkia breweri
S-adenosyl-L-homocysteine + methyleugenol
-
485153
Clarkia breweri
?
2.1.1.146
S-adenosyl-L-methionine + isoeugenol
-
485153
Clarkia breweri
S-adenosyl-L-homocysteine + isomethyleugenol
-
485153
Clarkia breweri
?
Subunits
EC Number
Subunits
Commentary
Organism
2.1.1.146
dimer
2 * 40000, SDS-PAGE
Clarkia breweri
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
2.1.1.146
35
-
30 min, stable up to
Clarkia breweri
2.1.1.146
45
-
30 min, activity is dramatically decreased
Clarkia breweri
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.146
7.5
-
methylation of eugenol and isoeugenol
Clarkia breweri
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.146
expression in Escherichia coli
Clarkia breweri
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.146
Ca2+
5 mM, weak inhibition
Clarkia breweri
2.1.1.146
Cu2+
5 mM, strong inhibition
Clarkia breweri
2.1.1.146
Fe2+
5 mM, strong inhibition
Clarkia breweri
2.1.1.146
Fe3+
5 mM, strong inhibition
Clarkia breweri
2.1.1.146
K+
5 mM, weak inhibition
Clarkia breweri
2.1.1.146
Mg2+
5 mM, 33% inhibition
Clarkia breweri
2.1.1.146
Mn2+
5 mM, strong inhibition
Clarkia breweri
2.1.1.146
Na+
5 mM, weak inhibition
Clarkia breweri
2.1.1.146
NH4+
5 mM, weak inhibition
Clarkia breweri
2.1.1.146
Zn2+
5 mM, strong inhibition
Clarkia breweri
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.146
0.007
-
eugenol
plant-purified enzyme
Clarkia breweri
2.1.1.146
0.019
-
S-adenosyl-L-methionine
recombinant enzyme
Clarkia breweri
2.1.1.146
0.027
-
S-adenosyl-L-methionine
plant-purified enzyme
Clarkia breweri
2.1.1.146
0.03
-
eugenol
recombinant enzyme
Clarkia breweri
2.1.1.146
0.058
-
isoeugenol
plant-purified enzyme
Clarkia breweri
2.1.1.146
0.074
-
isoeugenol
recombinant enzyme
Clarkia breweri
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.1.1.146
additional information
methylation of eugenol and isoeugenol
Clarkia breweri
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.146
40000
-
2 * 40000, SDS-PAGE
Clarkia breweri
2.1.1.146
88000
-
gel filtration
Clarkia breweri
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.146
-
Clarkia breweri
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.146
flower
-
Clarkia breweri
-
2.1.1.146
petal
-
Clarkia breweri
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.1.1.146
additional information
-
-
Clarkia breweri
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.1.1.146
S-adenosyl-L-methionine + eugenol
-
485153
Clarkia breweri
S-adenosyl-L-homocysteine + methyleugenol
-
485153
Clarkia breweri
?
2.1.1.146
S-adenosyl-L-methionine + isoeugenol
-
485153
Clarkia breweri
S-adenosyl-L-homocysteine + isomethyleugenol
-
485153
Clarkia breweri
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.1.146
dimer
2 * 40000, SDS-PAGE
Clarkia breweri
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
2.1.1.146
35
-
30 min, stable up to
Clarkia breweri
2.1.1.146
45
-
30 min, activity is dramatically decreased
Clarkia breweri
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.146
7.5
-
methylation of eugenol and isoeugenol
Clarkia breweri