BRENDA - Enzyme Database show

Alcohol oxidase from Candida boidinii

Sahm, H.; Schuette, H.; Kula, M.R.; Methods Enzymol. 89, 424-428 (1982)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.3.13
Cu2+
1 mM, 95% inhibition
Candida boidinii
1.1.3.13
PCMB
1 mM, complete inhibition
Candida boidinii
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.3.13
microbody
-
Candida boidinii
42579
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.3.13
additional information
no metal requirement
Candida boidinii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.13
74000
-
8 * 74000, SDS-PAGE
Candida boidinii
1.1.3.13
600000
-
equilibrium sedimentation
Candida boidinii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.3.13
methanol + O2
Candida boidinii
key enzyme of methanol metabolism
formaldehyde + H2O2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.3.13
Candida boidinii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.3.13
-
Candida boidinii
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.3.13
10.94
-
-
Candida boidinii
Storage Stability
EC Number
Storage Stability
Organism
1.1.3.13
-20°C, stable
Candida boidinii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.3.13
2-buten-1-ol + O2
-
484912
Candida boidinii
2-buten-1-al + H2O2
-
-
-
?
1.1.3.13
2-propen-1-ol + O2
-
484912
Candida boidinii
2-propen-1-al + H2O2
-
-
-
?
1.1.3.13
chloroethanol + O2
-
484912
Candida boidinii
chloroethanal + H2O2
-
-
-
?
1.1.3.13
ethanol + O2
-
484912
Candida boidinii
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
formaldehyde + O2
-
484912
Candida boidinii
formate + H2O2
-
484912
Candida boidinii
?
1.1.3.13
methanol + O2
-
484912
Candida boidinii
formaldehyde + H2O2
-
484912
Candida boidinii
?
1.1.3.13
methanol + O2
key enzyme of methanol metabolism
484912
Candida boidinii
formaldehyde + H2O2
-
-
-
?
1.1.3.13
n-butanol + O2
-
484912
Candida boidinii
n-butanal + H2O2
-
-
-
?
1.1.3.13
n-propanol + O2
-
484912
Candida boidinii
n-propanal + H2O2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.3.13
octamer
8 * 74000, SDS-PAGE
Candida boidinii
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.3.13
30
-
-
Candida boidinii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.13
7.5
9.5
-
Candida boidinii
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.3.13
7
10
stable
Candida boidinii
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.13
FAD
1 mol FAD per mol of subunit
Candida boidinii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.13
FAD
1 mol FAD per mol of subunit
Candida boidinii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.3.13
Cu2+
1 mM, 95% inhibition
Candida boidinii
1.1.3.13
PCMB
1 mM, complete inhibition
Candida boidinii
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.3.13
microbody
-
Candida boidinii
42579
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.3.13
additional information
no metal requirement
Candida boidinii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.13
74000
-
8 * 74000, SDS-PAGE
Candida boidinii
1.1.3.13
600000
-
equilibrium sedimentation
Candida boidinii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.3.13
methanol + O2
Candida boidinii
key enzyme of methanol metabolism
formaldehyde + H2O2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.3.13
-
Candida boidinii
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.3.13
10.94
-
-
Candida boidinii
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.3.13
-20°C, stable
Candida boidinii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.3.13
2-buten-1-ol + O2
-
484912
Candida boidinii
2-buten-1-al + H2O2
-
-
-
?
1.1.3.13
2-propen-1-ol + O2
-
484912
Candida boidinii
2-propen-1-al + H2O2
-
-
-
?
1.1.3.13
chloroethanol + O2
-
484912
Candida boidinii
chloroethanal + H2O2
-
-
-
?
1.1.3.13
ethanol + O2
-
484912
Candida boidinii
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
formaldehyde + O2
-
484912
Candida boidinii
formate + H2O2
-
484912
Candida boidinii
?
1.1.3.13
methanol + O2
-
484912
Candida boidinii
formaldehyde + H2O2
-
484912
Candida boidinii
?
1.1.3.13
methanol + O2
key enzyme of methanol metabolism
484912
Candida boidinii
formaldehyde + H2O2
-
-
-
?
1.1.3.13
n-butanol + O2
-
484912
Candida boidinii
n-butanal + H2O2
-
-
-
?
1.1.3.13
n-propanol + O2
-
484912
Candida boidinii
n-propanal + H2O2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.3.13
octamer
8 * 74000, SDS-PAGE
Candida boidinii
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.3.13
30
-
-
Candida boidinii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.13
7.5
9.5
-
Candida boidinii
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.3.13
7
10
stable
Candida boidinii