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Literature summary extracted from

  • Bringer, S.; Sprey, B.; Sahm, H.
    Purification and properties of alcohol oxidase from Poria contigua (1979), Eur. J. Biochem., 101, 563-570.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.3.13 freezing at -20°C and thawing leads to 25% loss of activity Poria contigua

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.13 NaN3 strong Poria contigua
1.1.3.13 PCMB 1 mM, complete inhibition Poria contigua

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.13 0.2
-
 methanol
-
 Poria contigua
1.1.3.13 1
-
 ethanol
-
 Poria contigua
1.1.3.13 3.2
-
 2-propin-1-ol
-
 Poria contigua
1.1.3.13 6.1
-
 formaldehyde
-
 Poria contigua
1.1.3.13 8.3
-
 n-Propanol
-
 Poria contigua
1.1.3.13 21.3
-
 n-butanol
-
 Poria contigua
1.1.3.13 25
-
 Isopropanol
-
 Poria contigua
1.1.3.13 27.2
-
 2-mercaptoethanol
-
 Poria contigua

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.13 cytoplasm
-
 Poria contigua 5737
-
1.1.3.13 microbody
-
 Poria contigua 42579
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.13 additional information no iron cofactor Poria contigua

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.13 79000
-
 8 * 79000, SDS-PAGE Poria contigua
1.1.3.13 610000
-
 equilibrium sedimentation Poria contigua

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.13 methanol + O2 Poria contigua key enzyme of methanol metabolism formaldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.13 Poria contigua
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.13
-
 Poria contigua

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.13 mycelium
-
 Poria contigua
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.13 20.7
-
-
 Poria contigua

Storage Stability

EC Number Storage Stability Organism
1.1.3.13 4°C, purified, stable for 2 weeks Poria contigua
1.1.3.13 4°C, storage in presence of antimicrobial agent sodium azide, 0.01% in 50 mM potassium phosphate buffer, pH 7.7, stable for at least 2 weeks Poria contigua

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.13 2-mercaptoethanol + O2
-
 Poria contigua 2-mercaptoethanal + H2O2
-
 ?
1.1.3.13 2-propyn-1-ol + O2
-
 Poria contigua propyn-1-al + H2O2
-
 ?
1.1.3.13 2-propyn-1-ol + O2
-
 Poria contigua 2-propyn-1-al + H2O2
-
 ?
1.1.3.13 ethanol + O2
-
 Poria contigua acetaldehyde + H2O2
-
 ?
1.1.3.13 formaldehyde + O2
-
 Poria contigua formate + H2O2
-
 ?
1.1.3.13 isopropanol + O2
-
 Poria contigua 2-propanone + H2O2
-
 ?
1.1.3.13 methanol + O2
-
 Poria contigua formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2 key enzyme of methanol metabolism Poria contigua formaldehyde + H2O2
-
 ?
1.1.3.13 n-butanol + O2
-
 Poria contigua n-butanal + H2O2
-
 ?
1.1.3.13 n-propanol + O2
-
 Poria contigua n-propanal + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.13 octamer 8 * 79000, SDS-PAGE Poria contigua

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.13 FAD 1 mol FAD per mol of subunit Poria contigua

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.3.13 0.02
-
 Sodium azide
-
 Poria contigua