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Literature summary extracted from

  • Cymeryng, C.; Cazzulo, J.J.; Cannata, J.J.B.
    Phosphoenolpyruvate carboxykinase from Trypanosoma cruzi. Purification and physicochemical and kinetic properties (1995), Mol. Biochem. Parasitol., 73, 91-101.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.49 5,5'-dithiobis(2-nitrobenzoate)
-
 Trypanosoma cruzi
4.1.1.49 Fluorescein mercuric acetate
-
 Trypanosoma cruzi
4.1.1.49 IDP inhibits exchange reaction with ATP or GTP Trypanosoma cruzi
4.1.1.49 o-Iodosobenzoate 0.5 mM, 50% inhibition Trypanosoma cruzi
4.1.1.49 p-chloromercuribenzenesulfonate
-
 Trypanosoma cruzi
4.1.1.49 p-chloromercuribenzoate
-
 Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.49 0.017
-
 ADP ADP in form of MnADP- Trypanosoma cruzi
4.1.1.49 0.019
-
 ATP exchange reaction Trypanosoma cruzi
4.1.1.49 0.019
-
 ATP ATP in form of MnATP2- Trypanosoma cruzi
4.1.1.49 0.027
-
 ATP ATP in form of MnATP2- Trypanosoma cruzi
4.1.1.49 0.035
-
 phosphoenolpyruvate
-
 Trypanosoma cruzi
4.1.1.49 0.041
-
 oxaloacetate exchange reaction Trypanosoma cruzi
4.1.1.49 0.044
-
 oxaloacetate
-
 Trypanosoma cruzi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.49 Co2+ exchange reaction: Mn2+ is the most effective divalent cation activator followed by Cd2+ and Mg2+ Trypanosoma cruzi
4.1.1.49 Mg2+ exchange reaction: MnCl2 is the most effective divalent cation activator followed by Cd2+ and Mg2+ Trypanosoma cruzi
4.1.1.49 Mn2+ exchange reaction: Mn2+ is the most effective divalent cation activator followed by Cd2+ and Mg2+ Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.49 Trypanosoma cruzi
-
 Tul O strain
-
4.1.1.49 Trypanosoma cruzi Tul O
-
 Tul O strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.49
-
 Trypanosoma cruzi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.49 epimastigote
-
 Trypanosoma cruzi
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.49 additional information
-
-
 Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.49 ADP + phosphoenolpyruvate + CO2
-
 Trypanosoma cruzi ATP + oxaloacetate
-
 ?
4.1.1.49 ADP + phosphoenolpyruvate + CO2
-
 Trypanosoma cruzi Tul O ATP + oxaloacetate
-
 ?
4.1.1.49 ATP + oxaloacetate
-
 Trypanosoma cruzi ADP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.49 ATP + oxaloacetate
-
 Trypanosoma cruzi Tul O ADP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.49 CTP + oxaloacetate 15% of the activity with ATP Trypanosoma cruzi CDP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.49 GTP + oxaloacetate 52% of the activity with ATP Trypanosoma cruzi GDP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.49 GTP + oxaloacetate 52% of the activity with ATP Trypanosoma cruzi Tul O GDP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.49 ITP + oxaloacetate 36% of the activity with ATP Trypanosoma cruzi IDP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.49 ITP + oxaloacetate 36% of the activity with ATP Trypanosoma cruzi Tul O IDP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.49 additional information CO2-exchange reaction with ATP, GTP, ITP, CTP, UTP and TTP Trypanosoma cruzi ?
-
 ?
4.1.1.49 additional information CO2-exchange reaction with ATP, GTP, ITP, CTP, UTP and TTP Trypanosoma cruzi Tul O ?
-
 ?
4.1.1.49 TTP + oxaloacetate 16% of the activity with ATP Trypanosoma cruzi TDP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.49 UTP + oxaloacetate 20% of the activity with ATP Trypanosoma cruzi UDP + phosphoenolpyruvate + CO2
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.49 0.5
-
 o-Iodosobenzoate
-
 Trypanosoma cruzi