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Literature summary extracted from

  • Holuigue, L.; Herrera, L.; Phillips, O.M.; Young, M.; Alende, J.E.
    CO2 fixation by mineral-leaching bacteria: characteristics of the ribulose bisphosphate carboxylase-oxygenase of Thiobacilllus ferrooxidans (1987), Biotechnol. Appl. Biochem., 9, 497-505.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.39 6-phosphogluconate
-
 Acidithiobacillus ferrooxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.39 0.028
-
 CO2
-
 Acidithiobacillus ferrooxidans
4.1.1.39 0.08
-
 D-ribulose 1,5-bisphosphate
-
 Acidithiobacillus ferrooxidans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.39 cytoplasm
-
 Acidithiobacillus ferrooxidans 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.39 15500
-
 A8B8, 8 * 54000 (A) + 8 * 15500 (B), SDS-PAGE Acidithiobacillus ferrooxidans
4.1.1.39 54000
-
 A8B8, 8 * 54000 (A) + 8 * 15500 (B), SDS-PAGE Acidithiobacillus ferrooxidans
4.1.1.39 570000
-
 non-denaturing gel electrophoresis Acidithiobacillus ferrooxidans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.39 D-Ribulose 1,5-bisphosphate + CO2 Acidithiobacillus ferrooxidans
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.39 Acidithiobacillus ferrooxidans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.39 partial Acidithiobacillus ferrooxidans

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.1.39 2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O D-ribulose 1,5-bisphosphate + CO2 = 2 3-phospho-D-glycerate, carboxylase reaction Acidithiobacillus ferrooxidans
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.39 112
-
 carboxylase Acidithiobacillus ferrooxidans

Storage Stability

EC Number Storage Stability Organism
4.1.1.39 4°C, 70% (NH4)2SO4 Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.39 D-Ribulose 1,5-bisphosphate + CO2
-
 Acidithiobacillus ferrooxidans D-3-Phosphoglycerate
-
 ?
4.1.1.39 D-Ribulose 1,5-bisphosphate + CO2
-
 Acidithiobacillus ferrooxidans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.39 hexadecamer A8B8, 8 * 54000 (A) + 8 * 15500 (B), SDS-PAGE Acidithiobacillus ferrooxidans