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Literature summary extracted from

  • Wolff, D.J.; Lubeskie, A.; Umansky, S.
    The inhibition of the constitutive bovine endothelial nitric oxide synthase by imidazole and indazole agents (1994), Arch. Biochem. Biophys., 314, 360-366.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.39 dithiothreitol
-
 Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.39 1-phenylimidazole reversible inhibition of endothelial enzyme, competitive versus L-arginine and tetrahydrobiopterin, no inhibition of cytochrome c reduction Bos taurus
1.14.13.39 7-nitroindazole reversible inhibition of endothelial enzyme, competitive versus tetrahydrobiopterin, no inhibition of cytochrome c reduction Bos taurus
1.14.13.39 imidazole inhibition of the endothelial enzyme, competitive versus L-arginine, no inhibition of cytochrome c reduction Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Ca2+ required Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 endothelium constitutive, from pulmonary artery Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Bos taurus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 5,6,7,8-tetrahydro-L-biopterin stimulates Bos taurus
1.14.13.39 Calmodulin dependent on, endothelial enzyme Bos taurus
1.14.13.39 NADPH
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.13.39 0.0008
-
 7-nitroindazole
-
 Bos taurus
1.14.13.39 0.05
-
 imidazole
-
 Bos taurus
1.14.13.39 0.05
-
 1-phenylimidazole
-
 Bos taurus