Literature summary extracted from
Christiansen, J.; Goodwin, P.J.; Lanzilotta, W.N.; Seefeldt, L.C.; Dean, D.R.
Catalytic and biophysical properties of a nitrogenase Apo-MoFe protein produced by a nifB-deletion mutant of Azotobacter vinelandii (1998), Biochemistry, 37, 12611-12623.
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.18.6.1 |
additional information |
natural nifB deletion mutant, MoFe protein without FeMo-cofactor and with small changes in the electronic properties of the [4Fe-4S] cluster |
Azotobacter vinelandii |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.18.6.1 |
Iron |
contains [4Fe4S] cluster |
Azotobacter vinelandii |
|
1.18.6.1 |
Molybdenum |
- |
Azotobacter vinelandii |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.18.6.1 |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O |
Azotobacter vinelandii |
- |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.18.6.1 |
Azotobacter vinelandii |
- |
nitrogen fixation complex is encoded on nif gene cluster |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.18.6.1 |
FeMo-cofactorless MoFe protein from nifB deletion mutant |
Azotobacter vinelandii |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
1.18.6.1 |
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate |
enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein |
Azotobacter vinelandii |
|
1.18.6.1 |
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate |
schematic electron flow from Fe protein to substrate via MoFe protein and MoFe protein-cofactor |
Azotobacter vinelandii |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.18.6.1 |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O |
- |
Azotobacter vinelandii |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.18.6.1 |
More |
apo-MoFe protein has a alpha2beta2 subunit composition and interacts with Fe protein, can be rebuilt by addition of FeMo-cofactor |
Azotobacter vinelandii |