EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.18.6.1 | Iron | - |
Azotobacter vinelandii | |
1.18.6.1 | Mg2+ | Mg2+ required for MgATP complex | Azotobacter vinelandii | |
1.18.6.1 | Molybdenum | - |
Azotobacter vinelandii | |
1.19.6.1 | Iron | enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex | Azotobacter vinelandii | |
1.19.6.1 | Mg2+ | required for ATP hydrolysis | Azotobacter vinelandii | |
1.19.6.1 | Molybdenum | enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex | Azotobacter vinelandii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.18.6.1 | 63000 | - |
Fe protein | Azotobacter vinelandii |
1.18.6.1 | 230000 | - |
MoFe protein | Azotobacter vinelandii |
1.19.6.1 | 230000 | - |
nitrogenase MoFe protein component | Azotobacter vinelandii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.6.1 | 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Azotobacter vinelandii | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
1.19.6.1 | 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP | Azotobacter vinelandii | enzyme consists of 2 metalloproteins, Fe protein and MoFe protein, which are assumed to associate and dissociate to transfer a single electron to the substrates, termed Fe protein cycle, driven by MgATP hydrolysis, with the dissociation of the Fe protein-MoFe protein complex being the rate limiting step of the cycle | 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.18.6.1 | Azotobacter vinelandii | - |
- |
- |
1.19.6.1 | Azotobacter vinelandii | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.18.6.1 | 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | mechanism, Fe protein cycle | Azotobacter vinelandii | |
1.18.6.1 | 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme complex dissociation and association kinetics | Azotobacter vinelandii | |
1.18.6.1 | 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Azotobacter vinelandii | |
1.18.6.1 | 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | Fe protein and MoFe protein are assumed to associate and dissociate to transfer a single electron to the substrates | Azotobacter vinelandii | |
1.19.6.1 | 4 reduced flavodoxin + 2 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized flavodoxin + 2 NH4+ + H2 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: Fe protein and MoFe protein which are assumed to associate and dissociate to transfer a single electron to the substrates | Azotobacter vinelandii | |
1.19.6.1 | 4 reduced flavodoxin + 2 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized flavodoxin + 2 NH4+ + H2 + 16 ADP + 16 phosphate | mechanism, Fe protein cycle | Azotobacter vinelandii | |
1.19.6.1 | 4 reduced flavodoxin + 2 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized flavodoxin + 2 NH4+ + H2 + 16 ADP + 16 phosphate | enzyme complex dissociation and association kinetics | Azotobacter vinelandii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.6.1 | 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP | intermediate is a flavodoxin hydroquinone | Azotobacter vinelandii | 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate | - |
? | |
1.18.6.1 | 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Azotobacter vinelandii | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
1.18.6.1 | 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | slow enzyme | Azotobacter vinelandii | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
1.18.6.1 | 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Fe protein and MoFe protein are assumed to associate and dissociate to transfer a single electron to the substrates, termed Fe protein cycle, driven by MgATP hydrolysis, with the dissociation of the Fe protein-MoFe protein complex being the rate limiting step of the cycle | Azotobacter vinelandii | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
1.18.6.1 | dithionite + H+ + N2 + ATP | in vitro substrate | Azotobacter vinelandii | ? | - |
? | |
1.18.6.1 | flavodoxin hydroquinone + H+ + N2 + ATP + H2O | - |
Azotobacter vinelandii | ? | - |
? | |
1.19.6.1 | 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP | enzyme consists of 2 metalloproteins, Fe protein and MoFe protein, which are assumed to associate and dissociate to transfer a single electron to the substrates, termed Fe protein cycle, driven by MgATP hydrolysis, with the dissociation of the Fe protein-MoFe protein complex being the rate-limiting step of the cycle | Azotobacter vinelandii | 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate | i.e. flavodoxin semiquinone | ? | |
1.19.6.1 | 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP | slow enzyme | Azotobacter vinelandii | 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate | i.e. flavodoxin semiquinone | ? | |
1.19.6.1 | 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP | intermediate is a flavodoxin hydroquinone | Azotobacter vinelandii | 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate | i.e. flavodoxin semiquinone | ? | |
1.19.6.1 | 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP | enzyme consists of 2 metalloproteins, Fe protein and MoFe protein, which are assumed to associate and dissociate to transfer a single electron to the substrates, termed Fe protein cycle, driven by MgATP hydrolysis, with the dissociation of the Fe protein-MoFe protein complex being the rate limiting step of the cycle | Azotobacter vinelandii | 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate | - |
? | |
1.19.6.1 | dithionite + H+ + N2 + ATP | - |
Azotobacter vinelandii | ? | - |
? | |
1.19.6.1 | flavodoxin hydroquinone + H+ + N2 + ATP + H2O | - |
Azotobacter vinelandii | ? | - |
? | |
1.19.6.1 | additional information | dithionite can serve as reductant in vitro | Azotobacter vinelandii | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.18.6.1 | More | enzyme is composed of 2 metalloproteins: Fe protein and MoFe protein which are assumed to associate and dissociate to transfer a single electron to the substrates | Azotobacter vinelandii |
1.19.6.1 | dimer | 2 * alpha-beta-monomer | Azotobacter vinelandii |
1.19.6.1 | More | alpha-beta-monomer of the FeMo protein consists of the FeMo cofactor FeMoco with the substrate reduction site and the P-cluster | Azotobacter vinelandii |
1.19.6.1 | More | enzyme is composed of 2 metalloproteins: Fe protein and MoFe protein which are assumed to associate and dissociate to transfer a single electron to the substrates | Azotobacter vinelandii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.6.1 | 400 | - |
flavodoxin hydroquinone | before and after reduction of the nitrogenase complex relatively slow reactions take place, which limits the rate of the Fe protein cycle | Azotobacter vinelandii | |
1.19.6.1 | 400 | - |
flavodoxin hydroquinone | before and after reduction of the nitrogenase complex relative slow reactions take place, which limits the rate of the Fe protein cycle | Azotobacter vinelandii |