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Literature summary extracted from
- Characteristics of vellosimine reductase, a specific enzyme involved in the biosynthesis of the Rauwolfia alkaloid sarpagine (1984), Tetrahedron, 40, 1691-1699.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.273 | highly instable enzyme, in crude extracts, half-life of activity is 25 h at 4°C, 0.25% activity remains after 7 d, activity depends completely on the presence of 2-mercaptoethanol | Rauvolfia serpentina |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.273 | 1,10-phenanthroline | 1 mM, 71% inhibition | Rauvolfia serpentina |  |
| 1.1.1.273 | EDTA | 1 mM, 77% inhibition | Rauvolfia serpentina | |
| 1.1.1.273 | NADP+ | noncompetitive inhibition | Rauvolfia serpentina | |
| 1.1.1.273 | neocuproin | 1 mM, 65% inhibition | Rauvolfia serpentina | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.273 | 0.0167 | - |
vellosimine | - |
Rauvolfia serpentina | |
| 1.1.1.273 | 0.02 | - |
NADPH | - |
Rauvolfia serpentina | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.273 | 42000 | - |
- |
Rauvolfia serpentina |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.273 | Catharanthus roseus | - |
- |
- |
| 1.1.1.273 | no activity in Alstonia scholaris | - |
- |
- |
| 1.1.1.273 | no activity in Carissa macrocarpa | - |
- |
- |
| 1.1.1.273 | no activity in Catharanthus roseus | - |
- |
- |
| 1.1.1.273 | no activity in Coleus blumei | - |
- |
- |
| 1.1.1.273 | no activity in Malus sylvestris | - |
- |
- |
| 1.1.1.273 | no activity in Nicotiana sylvestris | - |
- |
- |
| 1.1.1.273 | no activity in Picralima nitida | - |
- |
- |
| 1.1.1.273 | no activity in Rhazya stricta | - |
- |
- |
| 1.1.1.273 | no activity in Stemmadenia tomentosa | - |
- |
- |
| 1.1.1.273 | no activity in Tabernanthe iboga | - |
- |
- |
| 1.1.1.273 | no activity in Voacanga africana | - |
- |
- |
| 1.1.1.273 | Rauvolfia serpentina | - |
- |
- |
| 1.1.1.273 | Rauvolfia verticillata | - |
- |
- |
| 1.1.1.273 | Rauvolfia vomitoria | - |
- |
- |
| 1.1.1.273 | Vinca herbacea | - |
- |
- |
| 1.1.1.273 | Vinca minor | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.273 | - |
Rauvolfia serpentina |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.273 | cell suspension culture | - |
Catharanthus roseus | - |
| 1.1.1.273 | cell suspension culture | - |
Rauvolfia vomitoria | - |
| 1.1.1.273 | cell suspension culture | - |
Rauvolfia serpentina | - |
| 1.1.1.273 | cell suspension culture | - |
Rauvolfia verticillata | - |
| 1.1.1.273 | cell suspension culture | - |
Vinca minor | - |
| 1.1.1.273 | cell suspension culture | - |
Vinca herbacea | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.273 | 0.00018 | - |
vellosimine reduction in cell suspension culture | Vinca herbacea |
| 1.1.1.273 | 0.0006 | - |
vellosimine reduction in cell suspension culture | Vinca minor |
| 1.1.1.273 | 0.0012 | - |
- |
Rauvolfia serpentina |
| 1.1.1.273 | 0.013 | - |
vellosimine reduction in cell suspension culture | Rauvolfia vomitoria |
| 1.1.1.273 | 0.0156 | - |
vellosimine reduction in cell suspension culture | Catharanthus roseus |
| 1.1.1.273 | 0.0192 | - |
vellosimine reduction in cell suspension culture | Rauvolfia verticillata |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.273 | 16-epi-vellosimine + NADPH | - |
Rauvolfia serpentina | ? | - |
? | |
| 1.1.1.273 | deoxyajmalal B + NADPH | - |
Rauvolfia serpentina | ? | - |
? | |
| 1.1.1.273 | gardneral + NADPH | - |
Rauvolfia serpentina | ? | - |
? | |
| 1.1.1.273 | geissoschizal + NADPH | - |
Rauvolfia serpentina | ? | - |
? | |
| 1.1.1.273 | N-methylgardneral + NADPH | - |
Rauvolfia serpentina | ? | - |
? | |
| 1.1.1.273 | vellosimine + NADPH | - |
Rauvolfia serpentina | 10-deoxysarpagine + NADP+ | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.273 | 7.6 | - |
- |
Rauvolfia serpentina |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.273 | 5 | 8.5 | 50% of maximal activity at pH 6.8 and pH 7.6 respectively | Rauvolfia serpentina |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.273 | NADPH | absolutely specific for NADPH | Rauvolfia serpentina | |
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