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Literature summary extracted from

  • Lloyd Raven, E.; Celik, A.; Cullis, P.M.; Sangar, R.; Sutcliffe, M.J.
    Engineering the active site of ascorbate peroxidase (2001), Biochem. Soc. Trans., 29, 105-111.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.11 recombinant enzyme Pisum sativum

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.11 W41A the mutation enables the efficient conversion of recombinant enzyme into a stereoselective oxidizing agent for sulfides Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.11 cytosol
-
 Pisum sativum 5829
-

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.11 Pisum sativum
-
 pea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.11 ethyl phenyl sulfide + H2O2
-
 Pisum sativum ? + H2O
-
 ?
1.11.1.11 isopropyl phenyl sulfide + H2O2
-
 Pisum sativum ? + H2O
-
 ?
1.11.1.11 L-ascorbate + H2O2
-
 Pisum sativum dehydroascorbate + H2O
-
 ?
1.11.1.11 methyl naphthalene sulfide + H2O2
-
 Pisum sativum ? + H2O
-
 ?
1.11.1.11 methyl phenyl sulfide + H2O2
-
 Pisum sativum ? + H2O
-
 ?
1.11.1.11 additional information experimental and modelled enantiomeric ratios R: S for oxidation of thioethers by recombinant enzyme and mutant Trp-41-Ala Pisum sativum ?
-
 ?
1.11.1.11 n-propyl phenyl sulfide + H2O2
-
 Pisum sativum ? + H2O
-
 ?
1.11.1.11 p-chlorophenyl methyl sulfide + H2O2
-
 Pisum sativum ? + H2O
-
 ?
1.11.1.11 p-cresol + cumene-hydroperoxide
-
 Pisum sativum 4a,9b-dihydro-8,9b-dimethyl-3(4H)-dibenzofuranone + 2,2'-dihydroxy-5,5'-dimethylbiphenyl + 1,1-dimethylbenzylalcohol + bis-(1-methyl-1-phenylethyl)peroxide the formation of bis-(1-methyl-1-phenylethyl)peroxide derives from the reaction of 1,1-dimethylbenzylalcohol with either p-cresol or 2,2'-dihydroxy-5,5'-dimethylbiphenyl ?
1.11.1.11 p-cresol + H2O2
-
 Pisum sativum 4a,9b-dihydro-8,9b-dimethyl-3(4H)-dibenzofuranone + 2,2'-dihydroxy-5,5'-dimethylbiphenyl + H2O these products, which are derived from reactions of the p-methylphenoxy radical, itself form as a direct result of single-electron oxidation of p-cresol by the enzyme, can be accommodated from the known chemistry of the radical products, the product ratio 4alpha,9beta-dihydro-8,9beta-dimethyl-3(4H)-dibenzofuranone: 2,2'-dihydroxy-5,5'-dimethylbiphenyl is found to depend on enzyme concentration ?
1.11.1.11 p-nitrophenyl methyl sulfide + H2O2
-
 Pisum sativum ? + H2O
-
 ?