EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.11.1.11 | crystals of Cys32Ser-mutant and DTNB-modified enzyme grown using the hanging drop, vapor diffusion method. The 2.0 A X-ray crystal structure of DTNB-modified enzyme shows clear electron density for the TNB group covalently attached to Cys32 in all four molecules of the asymmetric unit, indicating complete and specific modification | Pisum sativum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.11.1.11 | C32S | the mutation leads to approximately 70% drop in ascorbate peroxidase activity with no effect on guaiacol peroxidase activity, these results indicate that uncharged aromatic substrates and the anionic ascorbate molecule interact with different sites on the enzyme | Pisum sativum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.11 | 4.2 | - |
guaiacol | will-type enzyme | Pisum sativum | |
1.11.1.11 | 6.1 | - |
guaiacol | Cys32Ser mutant | Pisum sativum | |
1.11.1.11 | 9.7 | - |
guaiacol | DTNB-modified enzyme | Pisum sativum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.11 | Pisum sativum | - |
pea | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.11 | ferrocyanide + H2O2 | the Cys32Ser mutation has little effect on the kinetics of ferrocyanide turnover, but the DTNB modification decreases activity by approximately 90% at 300 mM ferrocyanide | Pisum sativum | ferricyanide + H2O | - |
? | |
1.11.1.11 | guaiacol + H2O2 | the DTNB-modified enzyme exhibits full activity | Pisum sativum | ? | - |
? | |
1.11.1.11 | L-ascorbate + H2O2 | the DTNB-modified enzyme exhibits only 1.3% wild-type activity when ascorbate is used as substrate, the DTNB-modified enzyme reacts normally with peroxide to give compound I but the rates of reduction of both compounds I and II by ascorbate are dramatically slowed. The Cys32Ser mutant has one-third wild-type activity. The ascorbate interactions with the enzyme are partly mediated through electrostatic interactions | Pisum sativum | dehydroascorbate + H2O | - |
? | |
1.11.1.11 | pyrogallol + H2O2 | the DTNB-modified enzyme exhibits full activity | Pisum sativum | 3-hydroxybenzo-1,2-quinone + H2O | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.11 | additional information | - |
additional information | substrate: guaiacol, effects of Cys32Ser mutagenesis and DTNB-modification | Pisum sativum | |
1.11.1.11 | 160 | - |
pyrogallol | wild-type, Cys32Ser mutant and DTNB-modified enzyme | Pisum sativum |