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Literature summary extracted from

  • Tran-Betcke, A.; Warnecke, U.; B๖cker, C.; Zaborosch, C.; Friedrich, B.
    Cloning and nucleotide sequences of the genes for the subunits of NAD-reducing hydrogenase of Alcaligenes eutrophus H16 (1990), J. Bacteriol., 172, 2920-2929.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.1.2
-
 Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.1.2 26000
-
 x * 63000 + x * 30000 + x * 26000 + x * 56000, SDS-PAGE, deduced from nucleotide sequence Cupriavidus necator
1.12.1.2 30000
-
 x * 63000 + x * 30000 + x * 26000 + x * 56000, SDS-PAGE, deduced from nucleotide sequence Cupriavidus necator
1.12.1.2 56000
-
 x * 63000 + x * 30000 + x * 26000 + x * 56000, SDS-PAGE, deduced from nucleotide sequence Cupriavidus necator
1.12.1.2 63000
-
 x * 63000 + x * 30000 + x * 26000 + x * 56000, SDS-PAGE, deduced from nucleotide sequence Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.1.2 H2 + NAD+ Cupriavidus necator utilization of H2 as energy source during autotrophic growth on hydrogen and CO2 H+ + NADH
-
 r
1.12.1.2 H2 + NAD+ Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 utilization of H2 as energy source during autotrophic growth on hydrogen and CO2 H+ + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.2 Cupriavidus necator
-
-
-
1.12.1.2 Cupriavidus necator
-
 formerly Alcaligenes eutrophus
-
1.12.1.2 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.2 H2 + NAD+
-
 Cupriavidus necator H+ + NADH
-
 ?
1.12.1.2 H2 + NAD+ utilization of H2 as energy source during autotrophic growth on hydrogen and CO2 Cupriavidus necator H+ + NADH
-
 r
1.12.1.2 H2 + NAD+
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 H+ + NADH
-
 ?
1.12.1.2 H2 + NAD+ utilization of H2 as energy source during autotrophic growth on hydrogen and CO2 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 H+ + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.12.1.2 ? x * 63000 + x * 30000 + x * 26000 + x * 56000, SDS-PAGE, deduced from nucleotide sequence Cupriavidus necator