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Literature summary extracted from

  • Van Dijk, C.; Mayhew, S.G.; Grande, H.J.; Veeger, C.
    Purification and properties of hydrogenase from Megasphaera elsdenii (1979), Eur. J. Biochem., 102, 317-330.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.12.7.2 MgCl2 up to 0.5 M, activation of methyl or benzyl viologen mediated hydrogen oxidation Megasphaera elsdenii
1.12.7.2 NaCl up to 0.5 M, activation of methyl or benzyl viologen mediated hydrogen oxidation Megasphaera elsdenii
1.12.7.2 Tris-HCl at pH 8, activation of methyl or benzyl viologen mediated hydrogen oxidation Megasphaera elsdenii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.7.2 5.7
-
 Oxidized benzyl viologen hydrogen oxidation Megasphaera elsdenii
1.12.7.2 171
-
 oxidized methyl viologen hydrogen oxidation Megasphaera elsdenii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.7.2 49000
-
 gel filtration Megasphaera elsdenii
1.12.7.2 52000
-
 SDS-PAGE Megasphaera elsdenii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.7.2 H2 + oxidized ferredoxin Megasphaera elsdenii
-
 H+ + reduced ferredoxin
-
 r
1.12.7.2 H2 + oxidized ferredoxin Megasphaera elsdenii LC1
-
 H+ + reduced ferredoxin
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Megasphaera elsdenii
-
 formerly Peptostreptococcus elsdenii
-
1.12.7.2 Megasphaera elsdenii LC1
-
 formerly Peptostreptococcus elsdenii
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2
-
 Megasphaera elsdenii

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.7.2 510
-
 reaction with methyl viologen Megasphaera elsdenii
1.12.7.2 1470
-
 after addition of 1 M NaCl, reaction with methyl viologen Megasphaera elsdenii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H2 + electron acceptor methyl viologen as electron acceptor Megasphaera elsdenii H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + electron acceptor benzyl viologen as electron acceptor Megasphaera elsdenii H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + electron acceptor methyl viologen as electron acceptor Megasphaera elsdenii LC1 H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + electron acceptor benzyl viologen as electron acceptor Megasphaera elsdenii LC1 H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + oxidized benzyl viologen
-
 Megasphaera elsdenii H+ + reduced benzyl viologen
-
 r
1.12.7.2 H2 + oxidized benzyl viologen
-
 Megasphaera elsdenii LC1 H+ + reduced benzyl viologen
-
 r
1.12.7.2 H2 + oxidized ferredoxin
-
 Megasphaera elsdenii H+ + reduced ferredoxin
-
 r
1.12.7.2 H2 + oxidized ferredoxin
-
 Megasphaera elsdenii LC1 H+ + reduced ferredoxin
-
 r
1.12.7.2 H2 + oxidized methyl viologen
-
 Megasphaera elsdenii H+ + reduced methyl viologen
-
 r
1.12.7.2 H2 + oxidized methyl viologen
-
 Megasphaera elsdenii LC1 H+ + reduced methyl viologen
-
 r