EC Number | Cloned (Comment) | Organism |
---|---|---|
1.12.7.2 | expressed in Escherichia coli | Tetradesmus obliquus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.12.7.2 | 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone | inhibition of H2 production, photosystem 1 is involved in the supply of electrons to the hydrogenase | Tetradesmus obliquus | |
1.12.7.2 | CO | strong inhibition | Tetradesmus obliquus | |
1.12.7.2 | O2 | strong inhibition | Tetradesmus obliquus | |
1.12.7.2 | sulfo-disalicylidinepropandiamine | hydrogenase activity is reduced up to 30-fold | Tetradesmus obliquus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.12.7.2 | 0.8 | - |
methyl viologen | - |
Tetradesmus obliquus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.12.7.2 | chloroplast | - |
Tetradesmus obliquus | 9507 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.12.7.2 | Fe | the catalytic site is an H-cluster, containing a [4Fe4S] subcluster and a [2Fe] center | Tetradesmus obliquus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.12.7.2 | 44000 | - |
1 * 44000, SDS-PAGE | Tetradesmus obliquus |
1.12.7.2 | 44500 | - |
gel filtration and SDS-PAGE | Tetradesmus obliquus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.7.2 | H2 + oxidized ferredoxin | Tetradesmus obliquus | ferredoxin links the enzyme to photosynthesis | H+ + reduced ferredoxin | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.12.7.2 | Tetradesmus obliquus | - |
wild-type Kützing 276-6 | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.12.7.2 | rapid inactivation under aerobic conditions | Tetradesmus obliquus |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.12.7.2 | strictly under anaerobic conditions | Tetradesmus obliquus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.12.7.2 | 700 | - |
hydrogen evolution | Tetradesmus obliquus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.7.2 | H2 + electron acceptor | methyl viologen as electron acceptor | Tetradesmus obliquus | H+ + reduced electron acceptor | - |
r | |
1.12.7.2 | H2 + oxidized ferredoxin | ferredoxin links the enzyme to photosynthesis | Tetradesmus obliquus | H+ + reduced ferredoxin | - |
r | |
1.12.7.2 | H2 + oxidized methyl viologen | - |
Tetradesmus obliquus | H+ + reduced methyl viologen | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.12.7.2 | monomer | 1 * 44000, SDS-PAGE | Tetradesmus obliquus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.7.2 | 60 | - |
- |
Tetradesmus obliquus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.12.7.2 | 7.3 | - |
- |
Tetradesmus obliquus |