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Literature summary extracted from
- Purification and molecular characterization of the H2 uptake membrane-bound NiFe-hydrogenase from the carboxidotrophic bacterium Oligotropha carboxidovorans (1997), J. Bacteriol., 179, 6053-6060.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.12.99.6 | - |
Afipia carboxidovorans |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.99.6 | Fe | 7.3 mol per enzyme | Afipia carboxidovorans |  |
| 1.12.99.6 | Ni | 0.725 mol per enzyme | Afipia carboxidovorans | |
| 1.12.99.6 | Sulfide | 7.2 mol labile sulfide per enzyme | Afipia carboxidovorans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.12.99.6 | 30000 | - |
1 * 69000 + 1 * 30000, SDS-PAGE | Afipia carboxidovorans |
| 1.12.99.6 | 69000 | - |
1 * 69000 + 1 * 30000, SDS-PAGE | Afipia carboxidovorans |
| 1.12.99.6 | 101700 | - |
calculated from amino acid sequence | Afipia carboxidovorans |
| 1.12.99.6 | 102000 | - |
sucrose density gradient centrifugation | Afipia carboxidovorans |
| 1.12.99.6 | 105000 | - |
gel filtration | Afipia carboxidovorans |
| 1.12.99.6 | 128000 | - |
native PAGE | Afipia carboxidovorans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.12.99.6 | Afipia carboxidovorans | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.12.99.6 | - |
Afipia carboxidovorans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.12.99.6 | 173 | 178 | - |
Afipia carboxidovorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.12.99.6 | H2 + acceptor | enzyme acts on artificial electron-accepting dyes, but is ineffective with pyridine nucleotides or other soluble physiological electron acceptors | Afipia carboxidovorans | H+ + reduced acceptor | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.12.99.6 | dimer | 1 * 69000 + 1 * 30000, SDS-PAGE | Afipia carboxidovorans |
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Release 2023.1 (January 2023)
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