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Literature summary extracted from
- The H2 sensor of Ralstonia eutropha. Biochemical characteristics, spectroscopic properties, and its interaction with a histidine protein kinase (2001), J. Biol. Chem., 276, 15592-15597.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.12.99.6 | 25 | - |
H2 | - |
Cupriavidus necator |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.12.99.6 | cytoplasm | - |
Cupriavidus necator | 5737 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.99.6 | Fe | 4Fe-4S cluster | Cupriavidus necator | |
| 1.12.99.6 | Ni | NiFe-hydrogenase | Cupriavidus necator | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.12.99.6 | 37000 | - |
alpha2,beta2, 2 * 37000 + 2 * 55000, SDS-PAGE | Cupriavidus necator |
| 1.12.99.6 | 55000 | - |
alpha2,beta2, 2 * 37000 + 2 * 55000, SDS-PAGE | Cupriavidus necator |
| 1.12.99.6 | 165000 | - |
gel filtration | Cupriavidus necator |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.12.99.6 | Cupriavidus necator | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 1.12.99.6 | enzyme is extremely sensitive to inactivation by O2 | Cupriavidus necator |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.12.99.6 | purified protein forms a complex with histidine protein kinase | Cupriavidus necator |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.12.99.6 | 0.942 | - |
- |
Cupriavidus necator |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.12.99.6 | 4°C, 20% loss of activity after 48 h under argon | Cupriavidus necator |
| 1.12.99.6 | 4°C, 50% loss of activity after 12 h under an atmosphere of 100% H2 | Cupriavidus necator |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.12.99.6 | H2 + acceptor | benzyl viologen acts as electron acceptor | Cupriavidus necator | H+ + reduced acceptor | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.12.99.6 | tetramer | alpha2,beta2, 2 * 37000 + 2 * 55000, SDS-PAGE | Cupriavidus necator |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.12.99.6 | 4 | - |
H2 production | Cupriavidus necator |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.12.99.6 | 5 | 10 | H2 uptake is constant over a pH range from pH 5 to pH 10 | Cupriavidus necator |
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