Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cerdan, P.; Rekik, M.; Harayama, S.
    Substrate specificity differences between two catechol 2,3-dioxygenases encoded by the TOL and NAH plasmids from Pseudomonas putida (1995), Eur. J. Biochem., 229, 113-118.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.2
-
 Escherichia coli
1.13.11.2
-
 Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.2 H250G reduced twofold relative kcat value for 3-methylcatechol Escherichia coli
1.13.11.2 H250G reduced twofold relative kcat value for 3-methylcatechol Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.2 3-methylcatechol suicide inhibition Escherichia coli
1.13.11.2 3-methylcatechol suicide inhibition Pseudomonas putida
1.13.11.2 4-methylcatechol suicide inhibition Escherichia coli
1.13.11.2 4-methylcatechol suicide inhibition Pseudomonas putida
1.13.11.2 catechol suicide inhibition Escherichia coli
1.13.11.2 catechol suicide inhibition Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 0.0014
-
 catechol
-
 Pseudomonas putida
1.13.11.2 0.0017
-
 4-methylcatechol
-
 Pseudomonas putida
1.13.11.2 0.0018
-
 3-methylcatechol
-
 Pseudomonas putida
1.13.11.2 0.0024
-
 3-methylcatechol
-
 Escherichia coli
1.13.11.2 0.004
-
 catechol
-
 Escherichia coli
1.13.11.2 0.006
-
 4-methylcatechol
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Escherichia coli
-
-
-
1.13.11.2 Escherichia coli JM83
-
-
-
1.13.11.2 Pseudomonas putida
-
 KT 2440
-
1.13.11.2 Pseudomonas putida KT 2440
-
 KT 2440
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.2
-
 Escherichia coli
1.13.11.2
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 3-methylcatechol + O2
-
 Escherichia coli 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 3-methylcatechol + O2
-
 Pseudomonas putida 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 3-methylcatechol + O2
-
 Pseudomonas putida KT 2440 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 3-methylcatechol + O2
-
 Escherichia coli JM83 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2
-
 Escherichia coli 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2
-
 Pseudomonas putida 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2
-
 Pseudomonas putida KT 2440 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2
-
 Escherichia coli JM83 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 catechol + O2
-
 Escherichia coli 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2
-
 Pseudomonas putida 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2
-
 Pseudomonas putida KT 2440 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2
-
 Escherichia coli JM83 2-hydroxymuconate semialdehyde
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.2 additional information
-
 additional information kcat values of enzyme and hybrid proteins Escherichia coli
1.13.11.2 additional information
-
 additional information kcat values of enzyme and hybrid proteins Pseudomonas putida
1.13.11.2 190
-
 3-methylcatechol
-
 Escherichia coli
1.13.11.2 490
-
 3-methylcatechol
-
 Pseudomonas putida
1.13.11.2 690
-
 catechol
-
 Escherichia coli
1.13.11.2 930
-
 catechol
-
 Pseudomonas putida
1.13.11.2 930
-
 4-methylcatechol
-
 Escherichia coli
1.13.11.2 930
-
 4-methylcatechol
-
 Pseudomonas putida