Literature summary extracted from
Riedel, A.; Fetzner, S.; Rampp, M.; Lingens, F.; Liebl, U.; Zimmermann, J.L.; Nitschke, W.
EPR, electron spin echo envelope modulation, and electron nuclear double resonance studies of the 2Fe2S centers of the 2-halobenzoate 1,2-dioxygenase from Burkholderia (Pseudomonas) cepacia 2CBS (1995), J. Biol. Chem., 270, 30869-30873.
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.14.12.13 |
Fe2+ |
iron atoms are coordinated by 4 cysteines and are bridged by a pair of acid-labile sulfur atoms in the 2Fe2S ferredoxins |
Burkholderia cepacia |
|
1.14.12.13 |
Sulfide |
iron atoms are coordinated by 4 cysteines and are bridged by a pair of acid-labile sulfur atoms in the 2Fe2S ferredoxins |
Burkholderia cepacia |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.14.12.13 |
2-chlorobenzoate + NADH + H+ + O2 |
Burkholderia cepacia |
- |
catechol + chloride + NAD+ + CO2 |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.12.13 |
Burkholderia cepacia |
- |
Burkholderia cepacia 2CBS, DSM 9959 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.12.13 |
gel filtration of oxygenase component |
Burkholderia cepacia |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
1.14.12.13 |
2-chlorobenzoate + NADH + H+ + O2 = catechol + chloride + NAD+ + CO2 |
EPR, electron spin echo envelope modulation, electron nuclear double resonance studies and the electrochemical potential are performed. Enzyme contains one ferredoxin-type (reductase) and one Rieske-type (oxygenase) 2Fe2S center |
Burkholderia cepacia |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.12.13 |
2-chlorobenzoate + NADH + H+ + O2 |
- |
Burkholderia cepacia |
catechol + chloride + NAD+ + CO2 |
- |
? |
|