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Literature summary extracted from

  • Batie, C.J.; Ballou, D.P.
    Phthalate dioxygenase (1990), Methods Enzymol., 188, 61-70.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.12.7 0.125
-
 O2
-
 Burkholderia cepacia
1.14.12.7 0.3
-
 Phthalate 25°C Burkholderia cepacia
1.14.12.7 0.5
-
 Phthalate 4°C Burkholderia cepacia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.7 Fe2+ one Fe2+ is bound at a mononuclear site Burkholderia cepacia
1.14.12.7 Iron two iron atoms are bound in a Rieske-type [2Fe-2S] center Burkholderia cepacia
1.14.12.7 additional information optimal activity at ionic strength of 80 mM Burkholderia cepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.7 48000
-
 4 * 48000, SDS-PAGE Burkholderia cepacia

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.7 Burkholderia cepacia
-
 DB01
-
1.14.12.7 Burkholderia cepacia
-
 two separate proteins are required for phthalate dioxygenase activity: phthalate dioxygenase and phthalate dioxygenase reductase
-
1.14.12.7 Burkholderia cepacia DB01
-
 DB01
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.7
-
 Burkholderia cepacia

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.12.7 phthalate + NADH + H+ + O2 = cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ reduced phthalate dioxygenase reductase first interacts with phthylate dioxygenase and reduces the Rieske [2Fe-2S]center. Phthalate and O2 then bind reversibly and reduced phthalate dioxygenase transfers one more electron to the enzyme Burkholderia cepacia
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.12.7 1.31
-
-
 Burkholderia cepacia

Storage Stability

EC Number Storage Stability Organism
1.14.12.7 4°C, pH 8.0, 100 mM HEPES buffer, 20% v/v glycerol, stable for 1 week Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.7 phthalate + NADH + O2
-
 Burkholderia cepacia cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ + H2O
-
 ?
1.14.12.7 phthalate + NADH + O2
-
 Burkholderia cepacia DB01 cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.12.7 tetramer 4 * 48000, SDS-PAGE Burkholderia cepacia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.12.7 21
-
 pH 6.8 or pH 8.0, 12 h stable Burkholderia cepacia
1.14.12.7 42
-
 1 h, stable Burkholderia cepacia
1.14.12.7 60
-
 5 min, stable Burkholderia cepacia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.12.7 6.8 8.2
-
 Burkholderia cepacia

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.12.7 6.8
-
 room temperature, 12 h, stable Burkholderia cepacia
1.14.12.7 8
-
 room temperature, 12 h, stable Burkholderia cepacia

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.7 NADH specific for NADH Burkholderia cepacia