EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.16.1 | lysolecithin | 1 mM, approx. 7.5fold stimulation of phosphorylated recombinant wild-type enzyme, S16E and S16D mutant enzymes, approx. 24fold stimulation of non-phosphorylated recombinant wild-type enzyme, S16A, S16Q, S16N and S16K mutant enzymes | Rattus norvegicus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.16.1 | expression of wild-type and S16E, S16Q, S16N, S16D, S16A, and S16K mutant enzyme in Escherichia coli | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.16.1 | S16A | similar Km for tetrahydrobiopterin and activity as wild-type | Rattus norvegicus |
1.14.16.1 | S16D | similar Km for tetrahydrobiopterin and activity as wild-type | Rattus norvegicus |
1.14.16.1 | S16E | slightly higher Km for tetrahydrobiopterin than wild-type, approx. 3fold higher Vmax with phenylalanine | Rattus norvegicus |
1.14.16.1 | S16K | similar Km for tetrahydrobiopterin and activity as wild-type | Rattus norvegicus |
1.14.16.1 | S16N | slightly higher Km for tetrahydrobiopterin than wild-type, approx. 3fold higher Vmax with phenylalanine | Rattus norvegicus |
1.14.16.1 | S16Q | slightly higher Km for tetrahydrobiopterin than wild-type, similar Vmax with phenylalanine | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.16.1 | 0.0025 | - |
tetrahydrobiopterin | S16A mutant enzyme | Rattus norvegicus | |
1.14.16.1 | 0.0027 | - |
tetrahydrobiopterin | S16K mutant enzyme | Rattus norvegicus | |
1.14.16.1 | 0.0028 | - |
tetrahydrobiopterin | S16D mutant enzyme | Rattus norvegicus | |
1.14.16.1 | 0.0034 | - |
tetrahydrobiopterin | S16E mutant enzyme | Rattus norvegicus | |
1.14.16.1 | 0.0036 | - |
tetrahydrobiopterin | S16N mutant enzyme | Rattus norvegicus | |
1.14.16.1 | 0.0044 | - |
tetrahydrobiopterin | S16Q mutant enzyme | Rattus norvegicus | |
1.14.16.1 | 0.0046 | - |
tetrahydrobiopterin | phosphorylated recombinant wild-type enzyme | Rattus norvegicus | |
1.14.16.1 | 0.025 | - |
tetrahydrobiopterin | recombinant wild-type enzyme | Rattus norvegicus | |
1.14.16.1 | 0.183 | - |
phenylalanine | S16E mutant enzyme | Rattus norvegicus | |
1.14.16.1 | 0.187 | - |
phenylalanine | phosphorylated recombinant wild-type enzyme | Rattus norvegicus | |
1.14.16.1 | 0.2 | - |
phenylalanine | recombinant wild-type enzyme | Rattus norvegicus | |
1.14.16.1 | 0.217 | - |
phenylalanine | S16Q mutant enzyme | Rattus norvegicus | |
1.14.16.1 | 0.254 | - |
phenylalanine | S16K mutant enzyme | Rattus norvegicus | |
1.14.16.1 | 0.266 | - |
phenylalanine | S16D mutant enzyme | Rattus norvegicus | |
1.14.16.1 | 0.287 | - |
phenylalanine | S16N mutant enzyme | Rattus norvegicus | |
1.14.16.1 | 0.288 | - |
phenylalanine | S16A mutant enzyme | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.16.1 | Rattus norvegicus | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.14.16.1 | side-chain modification | phosphorylated enzyme shows a 3.3fold increase in Vmax | Rattus norvegicus |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.16.1 | recombinant wild-type S16E, S16Q, S16N, S16D, S16A, and S16K mutant enzyme | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.16.1 | 4.9 | - |
recombinant wild-type enzyme | Rattus norvegicus |
1.14.16.1 | 5 | - |
phosphorylated recombinant wild-type enzyme, S16N and S16D mutant enzyme | Rattus norvegicus |
1.14.16.1 | 5.1 | - |
S16A and S16K mutant enzymes | Rattus norvegicus |
1.14.16.1 | 5.2 | - |
S16E and S16Q mutant enzyme | Rattus norvegicus |