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Literature summary extracted from

  • Kowlessur, D.; Yang, X.J.; Kaufman, S.
    Further studies of the role of Ser-16 in the regulation of the activity of phenylalanine hydroxylase (1995), Proc. Natl. Acad. Sci. USA, 92, 4743-4747.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.16.1 lysolecithin 1 mM, approx. 7.5fold stimulation of phosphorylated recombinant wild-type enzyme, S16E and S16D mutant enzymes, approx. 24fold stimulation of non-phosphorylated recombinant wild-type enzyme, S16A, S16Q, S16N and S16K mutant enzymes Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.16.1 expression of wild-type and S16E, S16Q, S16N, S16D, S16A, and S16K mutant enzyme in Escherichia coli Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.16.1 S16A similar Km for tetrahydrobiopterin and activity as wild-type Rattus norvegicus
1.14.16.1 S16D similar Km for tetrahydrobiopterin and activity as wild-type Rattus norvegicus
1.14.16.1 S16E slightly higher Km for tetrahydrobiopterin than wild-type, approx. 3fold higher Vmax with phenylalanine Rattus norvegicus
1.14.16.1 S16K similar Km for tetrahydrobiopterin and activity as wild-type Rattus norvegicus
1.14.16.1 S16N slightly higher Km for tetrahydrobiopterin than wild-type, approx. 3fold higher Vmax with phenylalanine Rattus norvegicus
1.14.16.1 S16Q slightly higher Km for tetrahydrobiopterin than wild-type, similar Vmax with phenylalanine Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.1 0.0025
-
tetrahydrobiopterin S16A mutant enzyme Rattus norvegicus
1.14.16.1 0.0027
-
tetrahydrobiopterin S16K mutant enzyme Rattus norvegicus
1.14.16.1 0.0028
-
tetrahydrobiopterin S16D mutant enzyme Rattus norvegicus
1.14.16.1 0.0034
-
tetrahydrobiopterin S16E mutant enzyme Rattus norvegicus
1.14.16.1 0.0036
-
tetrahydrobiopterin S16N mutant enzyme Rattus norvegicus
1.14.16.1 0.0044
-
tetrahydrobiopterin S16Q mutant enzyme Rattus norvegicus
1.14.16.1 0.0046
-
tetrahydrobiopterin phosphorylated recombinant wild-type enzyme Rattus norvegicus
1.14.16.1 0.025
-
tetrahydrobiopterin recombinant wild-type enzyme Rattus norvegicus
1.14.16.1 0.183
-
phenylalanine S16E mutant enzyme Rattus norvegicus
1.14.16.1 0.187
-
phenylalanine phosphorylated recombinant wild-type enzyme Rattus norvegicus
1.14.16.1 0.2
-
phenylalanine recombinant wild-type enzyme Rattus norvegicus
1.14.16.1 0.217
-
phenylalanine S16Q mutant enzyme Rattus norvegicus
1.14.16.1 0.254
-
phenylalanine S16K mutant enzyme Rattus norvegicus
1.14.16.1 0.266
-
phenylalanine S16D mutant enzyme Rattus norvegicus
1.14.16.1 0.287
-
phenylalanine S16N mutant enzyme Rattus norvegicus
1.14.16.1 0.288
-
phenylalanine S16A mutant enzyme Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.1 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.16.1 side-chain modification phosphorylated enzyme shows a 3.3fold increase in Vmax Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.1 recombinant wild-type S16E, S16Q, S16N, S16D, S16A, and S16K mutant enzyme Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.1 4.9
-
recombinant wild-type enzyme Rattus norvegicus
1.14.16.1 5
-
phosphorylated recombinant wild-type enzyme, S16N and S16D mutant enzyme Rattus norvegicus
1.14.16.1 5.1
-
S16A and S16K mutant enzymes Rattus norvegicus
1.14.16.1 5.2
-
S16E and S16Q mutant enzyme Rattus norvegicus