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Literature summary extracted from
- Purification of a peptidylglycine alpha-amidating enzyme from transplantable rat medullary thyroid carcinomas (1988), Arch. Biochem. Biophys., 261, 44-54.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.17.3 | 75000 | - |
gel filtration and native PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.17.3 | peptidylglycine + ascorbate + O2 | Rattus norvegicus | - |
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.17.3 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.17.3 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.17.3 | medullary thyroid carcinoma cell | transplantable | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.17.3 | 2.07 | - |
purified enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.17.3 | dansyl-D-Tyr-Val-Gly + ascorbate + O2 | - |
Rattus norvegicus | dansyl-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
| 1.14.17.3 | peptidylglycine + ascorbate + O2 | - |
Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
| 1.14.17.3 | peptidylglycine + ascorbate + O2 | COOH-terminal glycine | Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.17.3 | ? | x * 75000, SDS-PAGE | Rattus norvegicus |
| 1.14.17.3 | monomer | 1 * 75000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.17.3 | 37 | - |
assay at | Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.17.3 | 5 | - |
N-dansyl-Tyr-Val-Gly | - |
Rattus norvegicus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.17.3 | 5 | 5.5 | - |
Rattus norvegicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.17.3 | ascorbate | dependent on | Rattus norvegicus | |
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