BRENDA - Enzyme Database

Purification and characterization of recombinant squalene epoxidase

Nagumo, A.; Kamei, T.; Sakakibara, J.; Ono, T.; J. Lipid Res. 36, 1489-1497 (1995)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.14.17
additional information
105000 g supernatant, 8.5fold activation of microsomal enzyme, 3fold activation of N-terminal truncated recombinant enzyme
Rattus norvegicus
1.14.14.17
NADPH cytochrome c reductase
required for activity of N-terminal truncated recombinant enzyme in a concentration-dependent manner
Rattus norvegicus
1.14.14.17
Triton X-100
0.1%, 36fold activation of microsomal enzyme, 0.05%, 34fold activation of N-terminal truncated recombinant enzyme
Rattus norvegicus
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.14.14.17
expression of cDNA in Escherichia coli
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.17
NB-598
0.0000032 mM, 50% inhibition of microsomal enzyme, 0.0000019 mM, 50% inhibition of N-terminal truncated recombinant enzyme
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.17
0.0036
-
squalene
N-terminal truncated recombinant enzyme
Rattus norvegicus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.14.17
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.14.14.17
recombinant enzyme, Ni-NTA-agarose, Blue Sepharose CL-6B
Rattus norvegicus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.14.14.17
0.17
-
N-terminal truncated recombinant enzyme
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.14.17
2,3-oxidosqualene + NADPH + O2
N-terminal truncated recombinant enzyme
438206
Rattus norvegicus
2,3,22,23-dioxidosqualene + NADP+ + H2O
-
438206
Rattus norvegicus
?
1.14.14.17
squalene + NAD(P)H + O2
-
438206
Rattus norvegicus
(S)-squalene-2,3-epoxide + NADP+ + H2O
-
438206
Rattus norvegicus
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.14.17
7.5
8.5
microsomal and N-terminal truncated recombinant enzyme
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.17
FAD
required for activity
Rattus norvegicus
1.14.14.17
additional information
neither FMN nor riboflavin can replace FAD
Rattus norvegicus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.14.14.17
0.00000041
-
NB-598
N-terminal truncated recombinant enzyme
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.14.17
additional information
105000 g supernatant, 8.5fold activation of microsomal enzyme, 3fold activation of N-terminal truncated recombinant enzyme
Rattus norvegicus
1.14.14.17
NADPH cytochrome c reductase
required for activity of N-terminal truncated recombinant enzyme in a concentration-dependent manner
Rattus norvegicus
1.14.14.17
Triton X-100
0.1%, 36fold activation of microsomal enzyme, 0.05%, 34fold activation of N-terminal truncated recombinant enzyme
Rattus norvegicus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.17
expression of cDNA in Escherichia coli
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.17
FAD
required for activity
Rattus norvegicus
1.14.14.17
additional information
neither FMN nor riboflavin can replace FAD
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.17
NB-598
0.0000032 mM, 50% inhibition of microsomal enzyme, 0.0000019 mM, 50% inhibition of N-terminal truncated recombinant enzyme
Rattus norvegicus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.14.14.17
0.00000041
-
NB-598
N-terminal truncated recombinant enzyme
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.17
0.0036
-
squalene
N-terminal truncated recombinant enzyme
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.17
recombinant enzyme, Ni-NTA-agarose, Blue Sepharose CL-6B
Rattus norvegicus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.14.14.17
0.17
-
N-terminal truncated recombinant enzyme
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.14.17
2,3-oxidosqualene + NADPH + O2
N-terminal truncated recombinant enzyme
438206
Rattus norvegicus
2,3,22,23-dioxidosqualene + NADP+ + H2O
-
438206
Rattus norvegicus
?
1.14.14.17
squalene + NAD(P)H + O2
-
438206
Rattus norvegicus
(S)-squalene-2,3-epoxide + NADP+ + H2O
-
438206
Rattus norvegicus
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.14.17
7.5
8.5
microsomal and N-terminal truncated recombinant enzyme
Rattus norvegicus