BRENDA - Enzyme Database show

Inhibition of squalene epoxidase by allylamine antimycotic compounds. A comparative study of the fungal and mammalian enzymes

Ryder, N.S.; Dupont, M.C.; Biochem. J. 230, 765-770 (1985)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.17
(E)-N-(6,6-dimethylhept-2-en-4-ynyl)-N-methyl-1-naphthalenemethaneamine hydrochloride
i.e. compound SF 86-327, non-competitive inhibition
Candida albicans
1.14.14.17
(E)-N-(6,6-dimethylhept-2-en-4-ynyl)-N-methyl-1-naphthalenemethaneamine hydrochloride
i.e. compound SF 86-327, non-competitive inhibition
Candida parapsilosis
1.14.14.17
(E)-N-(6,6-dimethylhept-2-en-4-ynyl)-N-methyl-1-naphthalenemethaneamine hydrochloride
i.e. compound SF 86-327, non-competitive inhibition
Rattus norvegicus
1.14.14.17
(E)-N-methyl-N-(3-phenylprop-2-enyl)-1-naphthalenemethaneamine
i.e. naftifine, non-competitive inhibition
Candida albicans
1.14.14.17
(E)-N-methyl-N-(3-phenylprop-2-enyl)-1-naphthalenemethaneamine
i.e. naftifine, non-competitive inhibition
Candida parapsilosis
1.14.14.17
(E)-N-methyl-N-(3-phenylprop-2-enyl)-1-naphthalenemethaneamine
i.e. naftifine, non-competitive inhibition
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.17
0.011
-
squalene
-
Rattus norvegicus
1.14.14.17
0.016
-
squalene
-
Candida parapsilosis
1.14.14.17
0.05
-
squalene
-
Candida albicans
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.17
Candida albicans
-
-
-
1.14.14.17
Candida parapsilosis
-
-
-
1.14.14.17
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.17
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.17
squalene + NAD(P)H + O2
NADPH is preferred
438202
Rattus norvegicus
(S)-squalene-2,3-epoxide + NADP+ + H2O
-
438202
Rattus norvegicus
?
1.14.14.17
squalene + NAD(P)H + O2
NADH is preferred
438202
Candida albicans
(S)-squalene-2,3-epoxide + NADP+ + H2O
-
438202
Candida albicans
?
1.14.14.17
squalene + NAD(P)H + O2
NADH is preferred
438202
Candida parapsilosis
(S)-squalene-2,3-epoxide + NADP+ + H2O
-
438202
Candida parapsilosis
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.17
FAD
required for activity
Candida parapsilosis
1.14.14.17
FAD
required for activity
Candida albicans
1.14.14.17
FAD
required for activity
Rattus norvegicus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.14.14.17
0.00003
-
(E)-N-(6,6-dimethylhept-2-en-4-ynyl)-N-methyl-1-naphthalenemethaneamine hydrochloride
-
Candida albicans
1.14.14.17
0.00004
-
(E)-N-(6,6-dimethylhept-2-en-4-ynyl)-N-methyl-1-naphthalenemethaneamine hydrochloride
-
Candida parapsilosis
1.14.14.17
0.00034
-
(E)-N-methyl-N-(3-phenylprop-2-enyl)-1-naphthalenemethaneamine
-
Candida parapsilosis
1.14.14.17
0.0011
-
(E)-N-methyl-N-(3-phenylprop-2-enyl)-1-naphthalenemethaneamine
-
Candida albicans
1.14.14.17
0.077
-
(E)-N-(6,6-dimethylhept-2-en-4-ynyl)-N-methyl-1-naphthalenemethaneamine hydrochloride
-
Rattus norvegicus
1.14.14.17
1.44
-
(E)-N-methyl-N-(3-phenylprop-2-enyl)-1-naphthalenemethaneamine
-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.17
FAD
required for activity
Candida parapsilosis
1.14.14.17
FAD
required for activity
Candida albicans
1.14.14.17
FAD
required for activity
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.17
(E)-N-(6,6-dimethylhept-2-en-4-ynyl)-N-methyl-1-naphthalenemethaneamine hydrochloride
i.e. compound SF 86-327, non-competitive inhibition
Candida albicans
1.14.14.17
(E)-N-(6,6-dimethylhept-2-en-4-ynyl)-N-methyl-1-naphthalenemethaneamine hydrochloride
i.e. compound SF 86-327, non-competitive inhibition
Candida parapsilosis
1.14.14.17
(E)-N-(6,6-dimethylhept-2-en-4-ynyl)-N-methyl-1-naphthalenemethaneamine hydrochloride
i.e. compound SF 86-327, non-competitive inhibition
Rattus norvegicus
1.14.14.17
(E)-N-methyl-N-(3-phenylprop-2-enyl)-1-naphthalenemethaneamine
i.e. naftifine, non-competitive inhibition
Candida albicans
1.14.14.17
(E)-N-methyl-N-(3-phenylprop-2-enyl)-1-naphthalenemethaneamine
i.e. naftifine, non-competitive inhibition
Candida parapsilosis
1.14.14.17
(E)-N-methyl-N-(3-phenylprop-2-enyl)-1-naphthalenemethaneamine
i.e. naftifine, non-competitive inhibition
Rattus norvegicus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.14.14.17
0.00003
-
(E)-N-(6,6-dimethylhept-2-en-4-ynyl)-N-methyl-1-naphthalenemethaneamine hydrochloride
-
Candida albicans
1.14.14.17
0.00004
-
(E)-N-(6,6-dimethylhept-2-en-4-ynyl)-N-methyl-1-naphthalenemethaneamine hydrochloride
-
Candida parapsilosis
1.14.14.17
0.00034
-
(E)-N-methyl-N-(3-phenylprop-2-enyl)-1-naphthalenemethaneamine
-
Candida parapsilosis
1.14.14.17
0.0011
-
(E)-N-methyl-N-(3-phenylprop-2-enyl)-1-naphthalenemethaneamine
-
Candida albicans
1.14.14.17
0.077
-
(E)-N-(6,6-dimethylhept-2-en-4-ynyl)-N-methyl-1-naphthalenemethaneamine hydrochloride
-
Rattus norvegicus
1.14.14.17
1.44
-
(E)-N-methyl-N-(3-phenylprop-2-enyl)-1-naphthalenemethaneamine
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.17
0.011
-
squalene
-
Rattus norvegicus
1.14.14.17
0.016
-
squalene
-
Candida parapsilosis
1.14.14.17
0.05
-
squalene
-
Candida albicans
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.17
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.17
squalene + NAD(P)H + O2
NADPH is preferred
438202
Rattus norvegicus
(S)-squalene-2,3-epoxide + NADP+ + H2O
-
438202
Rattus norvegicus
?
1.14.14.17
squalene + NAD(P)H + O2
NADH is preferred
438202
Candida albicans
(S)-squalene-2,3-epoxide + NADP+ + H2O
-
438202
Candida albicans
?
1.14.14.17
squalene + NAD(P)H + O2
NADH is preferred
438202
Candida parapsilosis
(S)-squalene-2,3-epoxide + NADP+ + H2O
-
438202
Candida parapsilosis
?