Any feedback?
Literature summary extracted from
- Enzymatic synthesis of cytidine diphosphate 3,6-dideoxyhexoses. V. Partial purification of the two protein components required for introduction of the 3-deoxy group (1969), J. Biol. Chem., 244, 3598-3604.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.17.1.1 | additional information | a mutant which cannot synthesize CDP-abequose is found to be missing E1 | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.1 | 5,5'-dithiobis(2-nitrobenzoate) | 89% inhibition at 10 mM | Yersinia pseudotuberculosis |  |
| 1.17.1.1 | iodoacetamide | 85% inhibition at 100 mM | Yersinia pseudotuberculosis | |
| 1.17.1.1 | N-ethylmaleimide | 88% inhibition at 10 mM | Yersinia pseudotuberculosis | |
| 1.17.1.1 | p-chloromercuribenzoate | 91% inhibition at 1 mM | Yersinia pseudotuberculosis | |
| 1.17.1.1 | p-Chlorophenylsulfonate | 91% inhibition at 1 mM | Yersinia pseudotuberculosis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.17.1.1 | 0.15 | - |
CDP-4-keto-6-deoxy-D-glucose | - |
Yersinia pseudotuberculosis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.1 | additional information | no divalent cation required | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 1.17.1.1 | additional information | no divalent cation required | Yersinia pseudotuberculosis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.17.1.1 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
LT-2 | - |
| 1.17.1.1 | Yersinia pseudotuberculosis | - |
25 VO | - |
| 1.17.1.1 | Yersinia pseudotuberculosis 25VO | - |
25 VO | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.17.1.1 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 1.17.1.1 | enzyme E1: using filtration on a Sephadex G-100 column and column chromatography on DEAE-cellulose, enzyme E3: using two filtrations on Sephadex G-100 columns | Yersinia pseudotuberculosis |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.17.1.1 | CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O = CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H + H+ | mechanism | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 1.17.1.1 | CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O = CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H + H+ | mechanism | Yersinia pseudotuberculosis | |
| 1.17.1.1 | CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O = CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H + H+ | 2 proteins, E1 and E3, are involved but no partial reaction is observed in the presence of either alone | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 1.17.1.1 | CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O = CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H + H+ | 2 proteins, E1 and E3, are involved but no partial reaction is observed in the presence of either alone | Yersinia pseudotuberculosis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.1 | additional information | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 1.17.1.1 | additional information | - |
- |
Yersinia pseudotuberculosis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.1.1 | CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O | - |
? | |
| 1.17.1.1 | CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H | - |
Yersinia pseudotuberculosis | CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O | - |
? | |
| 1.17.1.1 | CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H | - |
Yersinia pseudotuberculosis 25VO | CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.17.1.1 | More | 2 proteins E1 and E3 are involved but no partial reaction has been observed in the presence of either alone | Salmonella enterica subsp. enterica serovar Typhimurium |
| 1.17.1.1 | More | 2 proteins E1 and E3 are involved but no partial reaction has been observed in the presence of either alone | Yersinia pseudotuberculosis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.1 | 7.3 | - |
- |
Yersinia pseudotuberculosis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.1 | 5.5 | 8.5 | pH 5.5: about 60% of activity maximum, pH 8.5: about 40% of activity maximum | Yersinia pseudotuberculosis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.1 | NADPH | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| 1.17.1.1 | NADPH | - |
Yersinia pseudotuberculosis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
