EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.20 | S-adenosylmethionine | stimulates | Arabidopsis sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.20 | two MTHFR genes, a cDNAs encoding functional MTHFR is cloned, sequenced and expressed in Saccharomyces cerevisiae | Zea mays |
1.5.1.20 | two MTHFR genes, two cDNAs encoding functional MTHFRs are cloned, sequenced and expressed in Saccharomyces cerevisiae at high levels | Arabidopsis sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.20 | additional information | not inhibited by S-adenosylmethionine | Arabidopsis sp. | |
1.5.1.20 | additional information | not inhibited by S-adenosylmethionine | Escherichia coli | |
1.5.1.20 | additional information | not inhibited by S-adenosylmethionine | Zea mays |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.5.1.20 | cytosol | - |
Arabidopsis sp. | 5829 | - |
1.5.1.20 | cytosol | - |
Zea mays | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.20 | 33000 | - |
4 * 33000 | Escherichia coli |
1.5.1.20 | 66000 | - |
2 * 66000, SDS-PAGE | Arabidopsis sp. |
1.5.1.20 | 66400 | - |
x * 66400, calculated from the nucleotide sequence | Zea mays |
1.5.1.20 | 141000 | - |
gel filtration | Arabidopsis sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.20 | 5,10-methylenetetrahydrofolate + NADH | Escherichia coli | - |
5-methyltetrahydrofolate + NAD+ | - |
? | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + NADH | Arabidopsis sp. | folate-mediated one-carbon metabolism | 5-methyltetrahydrofolate + NAD+ | - |
r | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + NADH | Zea mays | folate-mediated one-carbon metabolism | 5-methyltetrahydrofolate + NAD+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.20 | Arabidopsis sp. | - |
ecotype Columbia, expression in Saccharomyces cerevisiae | - |
1.5.1.20 | Escherichia coli | - |
- |
- |
1.5.1.20 | Zea mays | Q9SE94 | expression in Saccharomyces cerevisiae | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.20 | 643fold purification of recombinant enzyme, expressed in Saccharomyces cerevisiae | Arabidopsis sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.20 | leaf | - |
Arabidopsis sp. | - |
1.5.1.20 | leaf | - |
Zea mays | - |
1.5.1.20 | root | - |
Arabidopsis sp. | - |
1.5.1.20 | root | - |
Zea mays | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.20 | 6.92 | - |
recombinant enzyme | Arabidopsis sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.1.20 | on ice, 3 h, loss of about 50% of activity | Arabidopsis sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.20 | 5,10-methylenetetrahydrofolate + NADH | - |
Escherichia coli | 5-methyltetrahydrofolate + NAD+ | - |
? | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + NADH | folate-mediated one-carbon metabolism | Arabidopsis sp. | 5-methyltetrahydrofolate + NAD+ | - |
r | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + NADH | folate-mediated one-carbon metabolism | Zea mays | 5-methyltetrahydrofolate + NAD+ | - |
r | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | reverse reaction: menadione as electron acceptor | Escherichia coli | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | reverse reaction: menadione as electron acceptor | Arabidopsis sp. | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | reverse reaction: menadione as electron acceptor | Zea mays | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | prefers NADH as reductant | Escherichia coli | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: strong preference of NADH over NADPH | Arabidopsis sp. | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: strong preference of NADH over NADPH | Zea mays | 5-methyltetrahydrofolate + oxidized acceptor | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.20 | ? | x * 66400, calculated from the nucleotide sequence | Zea mays |
1.5.1.20 | dimer | 2 * 66000, SDS-PAGE | Arabidopsis sp. |
1.5.1.20 | homotetramer | 4 * 33000 | Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.20 | 37 | - |
assay at | Escherichia coli |
1.5.1.20 | 37 | - |
assay at | Arabidopsis sp. |
1.5.1.20 | 37 | - |
assay at | Zea mays |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.20 | NADH | - |
Escherichia coli | |
1.5.1.20 | NADH | strong preference of NADH over NADPH as reductant | Arabidopsis sp. | |
1.5.1.20 | NADH | strong preference of NADH over NADPH as reductant | Zea mays |