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Literature summary extracted from

  • Wilson, A.C.; Miller, R.W.
    Growth temperature-dependent stearoyl coenzyme A desaturase activity of Fusarium oxysporum microsomes (1978), Can. J. Biochem., 56, 1109-1114.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.19.1 1,10-phenanthroline 26% stimulation Fusarium oxysporum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.19.1 CN- 44% inhibition at 1 mM Fusarium oxysporum
1.14.19.1 cytochrome c 32% inhibition at 0.01 mM Fusarium oxysporum
1.14.19.1 EDTA 46% inhibition at 10 mM Fusarium oxysporum
1.14.19.1 Fe2+ 76% inhibition at 10 mM Fusarium oxysporum
1.14.19.1 menadione 66% inhibition at 0.1 mM Fusarium oxysporum
1.14.19.1 phenazine methosulfate 100% inhibition at 0.4 mM Fusarium oxysporum
1.14.19.1 Thenoyltrifluoroacetone 59% inhibition at 6 mM; i.e. 4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedione Fusarium oxysporum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.19.1 0.038
-
 NADPH
-
 Fusarium oxysporum
1.14.19.1 0.089
-
 NADH
-
 Fusarium oxysporum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.1 microsome
-
 Fusarium oxysporum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.19.1 Fe2+ iron-sulfur protein(s) may act as electron carrier(s) in place of the cytchrome b5 and NADH cytochrome b5 reductase system associated with the mammalian desaturase systems Fusarium oxysporum
1.14.19.1 superoxid dismutase 8% stimulation Fusarium oxysporum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.1 stearoyl-CoA + AH2 + O2 Fusarium oxysporum oleic acid biosynthesis oleoyl-CoA + A + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.1 Fusarium oxysporum
-
 f. sp. lycopersici
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.19.1 stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O presence of an unusual microsomal electron transport system associated with the desaturase Fusarium oxysporum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.19.1 additional information
-
 enzyme activity is highest in microsomes from cells grown at 37°C and lowest in cells grown at 15°C Fusarium oxysporum
1.14.19.1 0.00044
-
 enzyme of cells grown at 37°C Fusarium oxysporum

Storage Stability

EC Number Storage Stability Organism
1.14.19.1 -20°C, argon, microsomal fraction, stable for up to 4 weeks Fusarium oxysporum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.1 stearoyl-CoA + AH2 + O2 oleic acid biosynthesis Fusarium oxysporum oleoyl-CoA + A + H2O
-
 ?
1.14.19.1 stearoyl-CoA + NAD(P)H + O2
-
 Fusarium oxysporum oleoyl-CoA + NAD(P)+ + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.19.1 25
-
 assay at Fusarium oxysporum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.19.1 additional information
-
 repeated freezing causes loss of activity Fusarium oxysporum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.19.1 8.2 8.3 assay at pH 8.4 Fusarium oxysporum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.1 heme b-type, nonheme iron-sulfur component Fusarium oxysporum
1.14.19.1 NADH
-
 Fusarium oxysporum
1.14.19.1 NADPH absolute requirement, preferred Fusarium oxysporum