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Literature summary extracted from

  • Yoshida, T.; Tanaka, Y.; Mitsunaga, T.; Izumi, Y.
    CoASAc-independent phosphoenolpyruvate carboxylase from an obligate methylotroph Hyphomicrobium methylovorum GM2 - purification and characterization (1995), Biosci. Biotechnol. Biochem., 59, 140-142.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.31 Ag+
-
 Hyphomicrobium methylovorum
4.1.1.31 Asp
-
 Hyphomicrobium methylovorum
4.1.1.31 ATP
-
 Hyphomicrobium methylovorum
4.1.1.31 Cd2+
-
 Hyphomicrobium methylovorum
4.1.1.31 citrate
-
 Hyphomicrobium methylovorum
4.1.1.31 Cu2+
-
 Hyphomicrobium methylovorum
4.1.1.31 Fe2+
-
 Hyphomicrobium methylovorum
4.1.1.31 glycerate
-
 Hyphomicrobium methylovorum
4.1.1.31 glyoxylate
-
 Hyphomicrobium methylovorum
4.1.1.31 malate
-
 Hyphomicrobium methylovorum
4.1.1.31 Ni2+
-
 Hyphomicrobium methylovorum
4.1.1.31 PCMB
-
 Hyphomicrobium methylovorum
4.1.1.31 succinate
-
 Hyphomicrobium methylovorum
4.1.1.31 Zn2+
-
 Hyphomicrobium methylovorum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.31 Co2+ 18% of the activation with Mg2+ Hyphomicrobium methylovorum
4.1.1.31 Fe2+ 12% of the activation with Mg2+ Hyphomicrobium methylovorum
4.1.1.31 Mg2+ divalent cation required, Mg2+ is the best activator Hyphomicrobium methylovorum
4.1.1.31 Mn2+ 23% of the activation with Mg2+ Hyphomicrobium methylovorum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.31 88000
-
 4 * 88000, SDS-PAGE Hyphomicrobium methylovorum
4.1.1.31 389000
-
 gel filtration Hyphomicrobium methylovorum

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Hyphomicrobium methylovorum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31
-
 Hyphomicrobium methylovorum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.31 104
-
-
 Hyphomicrobium methylovorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 phosphoenolpyruvate + CO2
-
 Hyphomicrobium methylovorum phosphate + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.31 tetramer 4 * 88000, SDS-PAGE Hyphomicrobium methylovorum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.31 25
-
 30 min, pH 7, no loss of activity Hyphomicrobium methylovorum
4.1.1.31 30
-
 30 min, pH 7, 15% loss of activity Hyphomicrobium methylovorum
4.1.1.31 35
-
 30 min, 40% loss of activity Hyphomicrobium methylovorum
4.1.1.31 40
-
 30 min, pH 7, 89% loss of activity Hyphomicrobium methylovorum
4.1.1.31 45
-
 30 min, pH 7, complete loss of activity Hyphomicrobium methylovorum

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.31 acetyl-CoA no effect Hyphomicrobium methylovorum
4.1.1.31 NADPH activates Hyphomicrobium methylovorum