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Literature summary extracted from
- The metabolism of coumarin by a microorganism. V. Melilotate hydroxylase (1966), J. Biol. Chem., 241, 997-1003.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.4 | 2,2'-dipyridyl | - |
Arthrobacter sp. |  |
| 1.14.13.4 | FeSO4 | - |
Arthrobacter sp. | |
| 1.14.13.4 | KCN | - |
Arthrobacter sp. | |
| 1.14.13.4 | additional information | not inhibitory: iodoacetic acid, iodoacetamide | Arthrobacter sp. | |
| 1.14.13.4 | N-ethylmaleimide | - |
Arthrobacter sp. | |
| 1.14.13.4 | p-chloromercuribenzoate | - |
Arthrobacter sp. | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.4 | 0.086 | - |
melilotate | - |
Arthrobacter sp. | |
| 1.14.13.4 | 0.091 | - |
NADH | - |
Arthrobacter sp. | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.4 | 3-(2-hydroxyphenyl)propanoate + NADH + O2 | Arthrobacter sp. | i.e. melilotate, role in metabolism of coumarin | 3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.4 | Arthrobacter sp. | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.4 | partial | Arthrobacter sp. |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 1.14.13.4 | activity can be restored with FAD | Arthrobacter sp. |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.13.4 | -20°C, stable for 1 week | Arthrobacter sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.4 | 3-(2-hydroxyphenyl)propanoate + NADH + O2 | i.e. melilotate, reversibility could not be demonstrated | Arthrobacter sp. | 3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O | reversibility could not be demonstrated | ir | |
| 1.14.13.4 | 3-(2-hydroxyphenyl)propanoate + NADH + O2 | i.e. melilotate, role in metabolism of coumarin | Arthrobacter sp. | 3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O | - |
ir | |
| 1.14.13.4 | additional information | high specificity, low activity with 3-hydroxyphenylpropanoate with 20% of melilotate reduction, phenylpropanoate with 1-2% of meliotate reduction | Arthrobacter sp. | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.4 | 30 | - |
assay at | Arthrobacter sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.4 | 7 | 7.5 | - |
Arthrobacter sp. |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.4 | 5 | 9 | at pH 5.0 and 9.0: about 30% of maximum activity | Arthrobacter sp. |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.4 | FAD | flavoprotein | Arthrobacter sp. | |
| 1.14.13.4 | NADH | - |
Arthrobacter sp. | |
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Release 2023.1 (January 2023)
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