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Literature summary extracted from
- Purification and characterization of an oxygenase component in benzoate 1,2-dioxygenase system from Pseudomonas arvilla C-1 (1980), J. Biol. Chem., 255, 5058-5063.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.12.10 | 0.0039 | - |
benzoate | - |
Pseudomonas putida |  |
| 1.14.12.10 | 0.0043 | - |
O2 | - |
Pseudomonas putida | |
| 1.14.12.10 | 0.0048 | - |
NADH | - |
Pseudomonas putida | |
| 1.14.12.10 | 0.026 | - |
NADH-cytochrome c reductase component | - |
Pseudomonas putida |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.12.10 | Iron | 10 mol of iron per mol of enzyme, [2Fe-2S] clusters | Pseudomonas putida | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.10 | 37500 | - |
NADH-cytochrome c component | Pseudomonas putida |
| 1.14.12.10 | 270000 | - |
oxygenase component, gel filtration | Pseudomonas putida |
| 1.14.12.10 | 273000 | - |
oxygenase component, sedimentation equilibrium | Pseudomonas putida |
| 1.14.12.10 | 280000 | - |
oxygenase component, native PAGE | Pseudomonas putida |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.12.10 | Pseudomonas putida | - |
C-1 | - |
| 1.14.12.10 | Pseudomonas putida C-1 | - |
C-1 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.12.10 | oxygenase component | Pseudomonas putida |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.14.12.10 | benzoate + NADH + H+ + O2 = (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ | the enzyme consists of two proteins, a NADH-cytochrome c reductase and an oxygenase | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.10 | 4.9 | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.12.10 | 3-aminobenzoate + O2 + NADH | - |
Pseudomonas putida | 3-amino-2-hydro-1,2-dihydroxybenzoate | - |
? | |
| 1.14.12.10 | 3-aminobenzoate + O2 + NADH | - |
Pseudomonas putida C-1 | 3-amino-2-hydro-1,2-dihydroxybenzoate | - |
? | |
| 1.14.12.10 | 3-chlorobenzoate + O2 + NADH + H+ | - |
Pseudomonas putida | 3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
| 1.14.12.10 | 3-chlorobenzoate + O2 + NADH + H+ | - |
Pseudomonas putida C-1 | 3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
| 1.14.12.10 | 3-fluorobenzoate + O2 + NADH + H+ | benzoate analogues with substituents in meta position are oxygenated more rapidly than those with ortho- or para substitution | Pseudomonas putida | 3-fluoro-2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
| 1.14.12.10 | 3-fluorobenzoate + O2 + NADH + H+ | benzoate analogues with substituents in meta position are oxygenated more rapidly than those with ortho- or para substitution | Pseudomonas putida C-1 | 3-fluoro-2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
| 1.14.12.10 | 3-methylbenzoate + O2 + NADH + H+ | - |
Pseudomonas putida | 3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
| 1.14.12.10 | 4-fluorobenzoate + O2 + NADH | - |
Pseudomonas putida | 4-fluoro-2-hydro-1,2-dihydroxybenzoate | - |
? | |
| 1.14.12.10 | 4-fluorobenzoate + O2 + NADH | - |
Pseudomonas putida C-1 | 4-fluoro-2-hydro-1,2-dihydroxybenzoate | - |
? | |
| 1.14.12.10 | benzoate + NADH-cytochrome c reductase component + H+ + O2 | - |
Pseudomonas putida | (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+-cytochrome c reductase component | - |
r | |
| 1.14.12.10 | benzoate + O2 + NADH + H+ | - |
Pseudomonas putida | 2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
| 1.14.12.10 | benzoate + O2 + NADH + H+ | - |
Pseudomonas putida C-1 | 2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.10 | 24 | - |
assay at | Pseudomonas putida |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.12.10 | 367 | - |
benzoate | in the presence of saturating amounts of NADH-cytochrome c reductase | Pseudomonas putida | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.10 | 6.7 | - |
oxygenase component | Pseudomonas putida |
| 1.14.12.10 | 8.2 | - |
NADH-cytochrome c reductase component | Pseudomonas putida |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.10 | 6 | 8 | pH 6.0: about 55% activity, pH 8.0: about 60% activity | Pseudomonas putida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.12.10 | FAD | requirement, benzoate 1,2-dioxygenase system consists of 2 proteins: a 37500 Da NADH-cytochrome c reductase containing 1 FAD and 1 iron-sulfur cluster [2Fe-2S] and an oxygenase which may contain 4 [2Fe-2S] clusters | Pseudomonas putida | |
| 1.14.12.10 | NADH | requirement, benzoate 1,2-dioxygenase system consists of 2 proteins: a 37500 Da NADH-cytochrome c reductase containing 1 FAD and 1 iron-sulfur cluster [2Fe-2S] and an oxygenase which may contain 4 [2Fe-2S] clusters | Pseudomonas putida | |
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