BRENDA - Enzyme Database

Demonstration of 2-oxoglutarate:glyoxylate carboligase activity in Tetrahymena pyriformis

Zaror-Behrens, G.; Mavrides, C.; Can. J. Biochem. 51, 1505-1508 (1973)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.2.1.5
EDTA
-
Tetrahymena pyriformis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.2.1.5
mitochondrion
-
Tetrahymena pyriformis
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.2.1.5
Mg2+
-
Tetrahymena pyriformis
2.2.1.5
Mn2+
substitutes for Mg2+
Tetrahymena pyriformis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.2.1.5
Tetrahymena pyriformis
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.2.1.5
additional information
-
-
Tetrahymena pyriformis
2.2.1.5
0.128
-
maximum, cell-cycle-dependent
Tetrahymena pyriformis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.2.1.5
2-oxoglutarate + glyoxylate
-
395926
Tetrahymena pyriformis
2-hydroxy-3-oxohexandioate + CO2
i. e. 2-hydroxy-3-oxoadipic acid
395926
Tetrahymena pyriformis
-
2.2.1.5
2-oxoglutarate + glyoxylate
-
395926
Tetrahymena pyriformis
2-hydroxy-3-oxohexandioate + CO2
further spontaneous decarboxylation in the presence of acid
395926
Tetrahymena pyriformis
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.2.1.5
30
-
enzyme assay at
Tetrahymena pyriformis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.2.1.5
7
-
enzyme assay at
Tetrahymena pyriformis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.2.1.5
thiamine diphosphate
-
Tetrahymena pyriformis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.2.1.5
thiamine diphosphate
-
Tetrahymena pyriformis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.2.1.5
EDTA
-
Tetrahymena pyriformis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.2.1.5
mitochondrion
-
Tetrahymena pyriformis
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.2.1.5
Mg2+
-
Tetrahymena pyriformis
2.2.1.5
Mn2+
substitutes for Mg2+
Tetrahymena pyriformis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.2.1.5
additional information
-
-
Tetrahymena pyriformis
2.2.1.5
0.128
-
maximum, cell-cycle-dependent
Tetrahymena pyriformis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.2.1.5
2-oxoglutarate + glyoxylate
-
395926
Tetrahymena pyriformis
2-hydroxy-3-oxohexandioate + CO2
i. e. 2-hydroxy-3-oxoadipic acid
395926
Tetrahymena pyriformis
-
2.2.1.5
2-oxoglutarate + glyoxylate
-
395926
Tetrahymena pyriformis
2-hydroxy-3-oxohexandioate + CO2
further spontaneous decarboxylation in the presence of acid
395926
Tetrahymena pyriformis
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.2.1.5
30
-
enzyme assay at
Tetrahymena pyriformis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.2.1.5
7
-
enzyme assay at
Tetrahymena pyriformis