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Literature summary extracted from

  • Kuzuyama, T.; Takagi, M.; Takahashi, S.; Seto, H.
    Cloning and characterization of 1-deoxy-D-xylulose 5-phosphate synthase from Streptomyces sp. strain CL190, which uses both the mevalonate and nonmevalonate pathways for isopentenyl diphosphate biosynthesis (2000), J. Bacteriol., 182, 891-897.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.7 expressed in Escherichia coli Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.7 EDTA recombinant protein Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.7 0.065
-
 pyruvate recombinant protein Streptomyces sp.
2.2.1.7 0.096
-
 pyruvate
-
 Escherichia coli
2.2.1.7 0.12
-
 D-glyceraldehyde 3-phosphate recombinant protein Streptomyces sp.
2.2.1.7 0.24
-
 D-glyceraldehyde 3-phosphate
-
 Escherichia coli
2.2.1.7 35
-
 D-glyceraldehyde recombinant protein Streptomyces sp.
2.2.1.7 38
-
 D-glyceraldehyde
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.7 Mg2+
-
 Escherichia coli
2.2.1.7 Mg2+ recombinant protein, optimum concentration 1 mM Streptomyces sp.
2.2.1.7 Mn2+
-
 Escherichia coli
2.2.1.7 Mn2+ recombinant protein, optimum concentration 1 mM Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.7 60000
-
 2 * 60000, gel filtration Escherichia coli
2.2.1.7 65000 69000 SDS-PAGE Escherichia coli
2.2.1.7 68000
-
 predicted size from gene sequence Streptomyces sp.
2.2.1.7 70000
-
 recombinant protein, SDS-PAGE Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.7 Escherichia coli P77488
-
-
2.2.1.7 Streptomyces sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.7 overexpression Escherichia coli
2.2.1.7 recombinant protein, expressed in Escherichia coli Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.7 pyruvate + D-glyceraldehyde
-
 Streptomyces sp. 1-deoxy-D-xylulose + CO2
-
 ?
2.2.1.7 pyruvate + D-glyceraldehyde
-
 Escherichia coli 1-deoxy-D-xylulose + CO2
-
 ?
2.2.1.7 pyruvate + D-glyceraldehyde 3-phosphate
-
 Streptomyces sp. 1-deoxy-D-xylulose 5-phosphate + CO2
-
 ?
2.2.1.7 pyruvate + D-glyceraldehyde 3-phosphate
-
 Escherichia coli 1-deoxy-D-xylulose 5-phosphate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.7 dimer 2 * 65000-70000, gel filtration, native PAGE Streptomyces sp.
2.2.1.7 dimer 2 * 60000, gel filtration Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.7 42 44
-
 Escherichia coli
2.2.1.7 42 44 recombinant protein Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.7 7.5 8
-
 Escherichia coli
2.2.1.7 9
-
 recombinant protein Streptomyces sp.

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.7 thiamine diphosphate failure of DXP producting in the absence of thiamine diphosphate indicates its absolute requirement for the enzymatic reaction Streptomyces sp.