EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.52 | alpha-methyl-DL-tryptophan | can activate the enzyme when lower concentrations of L-Trp are used | Pseudomonas fluorescens | |
1.13.11.52 | ascorbic acid | the ferric enzyme is incapable of catalyzing the reaction but is reduced to an active ferrous form by the addition of ascorbic acid and L-Trp in presence of O2 | Pseudomonas fluorescens | |
1.13.11.52 | Cys | can reduce the inactive ferric form of the enzyme to the active ferrous form | Pseudomonas fluorescens | |
1.13.11.52 | DTT | can reduce the inactive ferric form of the enzyme to the active ferrous form | Pseudomonas fluorescens | |
1.13.11.52 | ethylhydroperoxide | can reduce the inactive ferric form of the enzyme to the active ferrous form | Pseudomonas fluorescens | |
1.13.11.52 | GSH | can reduce the inactive ferric form of the enzyme to the active ferrous form | Pseudomonas fluorescens | |
1.13.11.52 | H2O2 | can reduce the inactive ferric form of the enzyme to the active ferrous form | Pseudomonas fluorescens | |
1.13.11.52 | methylhydroperoxide | can reduce the inactive ferric form of the enzyme to the active ferrous form | Pseudomonas fluorescens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.52 | ferricyanide | - |
Pseudomonas fluorescens | |
1.13.11.52 | hydroxylamine | - |
Pseudomonas fluorescens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.52 | 0.06 | - |
O2 | - |
Pseudomonas fluorescens | |
1.13.11.52 | 0.3 | - |
L-Trp | - |
Pseudomonas fluorescens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.52 | copper | contains copper in significant quantity | Pseudomonas fluorescens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.52 | Pseudomonas fluorescens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.52 | - |
Pseudomonas fluorescens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.52 | 5.3 | - |
- |
Pseudomonas fluorescens |
EC Number | Storage Stability | Organism |
---|---|---|
1.13.11.52 | 0°C, under anaerobic conditions in presence of tryptophan, stable for several days | Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.52 | L-Trp + O2 | absolutely specific for L-Trp | Pseudomonas fluorescens | L-formylkynurenine | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.52 | 7.2 | - |
- |
Pseudomonas fluorescens |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.52 | 6.5 | - |
most stable at | Pseudomonas fluorescens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.52 | heme | contains protohematin IX as a prosthetic group | Pseudomonas fluorescens |