EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.146 | expression in Escherichia coli | Methanocaldococcus jannaschii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.146 | AMP | 10 mM, 76% inhibition | Methanocaldococcus jannaschii | |
2.7.1.146 | ATP | 10 mM, 47% inhibition | Methanocaldococcus jannaschii | |
2.7.1.146 | KCl | 500 mM, 16% inhibition | Methanocaldococcus jannaschii | |
2.7.1.146 | additional information | the addition of fructose 2,6-bisphosphate, pyruvate, glucose, phosphoenolpyruvate, or citrate to the assay mixture has no effect on the activity | Methanocaldococcus jannaschii | |
2.7.1.146 | NaCl | 500 mM, 12% inhibition | Methanocaldococcus jannaschii | |
2.7.1.146 | Zn2+ | no activity detected in presence of Zn2+ | Methanocaldococcus jannaschii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.146 | 0.0096 | - |
D-fructose 6-phosphate | pH 6.5, 50°C | Methanocaldococcus jannaschii | |
2.7.1.146 | 0.49 | - |
ADP | pH 6.5, 50°C | Methanocaldococcus jannaschii | |
2.7.1.146 | 11.9 | - |
acetyl-phosphate | pH 6.5, 50°C | Methanocaldococcus jannaschii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.146 | Ca2+ | - |
Methanocaldococcus jannaschii | |
2.7.1.146 | Ca2+ | activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+ | Methanocaldococcus jannaschii | |
2.7.1.146 | Co2+ | activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+ | Methanocaldococcus jannaschii | |
2.7.1.146 | Mg2+ | - |
Methanocaldococcus jannaschii | |
2.7.1.146 | Mg2+ | activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+ | Methanocaldococcus jannaschii | |
2.7.1.146 | Mn2+ | activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+ | Methanocaldococcus jannaschii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.146 | 50100 | - |
native PAGE | Methanocaldococcus jannaschii |
2.7.1.146 | 50100 | - |
non-denaturing PAGE | Methanocaldococcus jannaschii |
2.7.1.146 | 53362 | - |
1 * 53362, calculated from sequence | Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.146 | ADP + D-fructose 6-phosphate | Methanocaldococcus jannaschii | key enzyme of the glycolytic pathway | AMP + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.146 | ADP + D-fructose 6-phosphate | Methanocaldococcus jannaschii DSM 2661 | key enzyme of the glycolytic pathway | AMP + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.146 | D-fructose 6-phosphate + ADP | Methanocaldococcus jannaschii | ADP can be replaced by acetylphosphate | D-fructose 1,6-bisphosphate + AMP | - |
? | |
2.7.1.146 | D-fructose 6-phosphate + ADP | Methanocaldococcus jannaschii | ADP can be replaced by acetylphosphate | D-fructose 1,6-bisphosphate + AMP | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.146 | Methanocaldococcus jannaschii | - |
- |
- |
2.7.1.146 | Methanocaldococcus jannaschii | Q58999 | - |
- |
2.7.1.146 | Methanocaldococcus jannaschii DSM 2661 | Q58999 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.146 | - |
Methanocaldococcus jannaschii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.146 | 8.2 | - |
- |
Methanocaldococcus jannaschii |
2.7.1.146 | 8.2 | - |
pH 6.5, 50°C, direction of phosphorylation | Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.146 | acetyl-phosphate + D-fructose 6-phosphate | activity is 83% compared to the activity with ADP | Methanocaldococcus jannaschii | acetate + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.146 | acetyl-phosphate + D-fructose 6-phosphate | activity is 83% compared to the activity with ADP | Methanocaldococcus jannaschii DSM 2661 | acetate + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.146 | ADP + D-fructose 6-phosphate | key enzyme of the glycolytic pathway | Methanocaldococcus jannaschii | AMP + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.146 | ADP + D-fructose 6-phosphate | activity with GDP is 1.4% compared to the activity with ADP, activity with ATP is 0.3% compared to the activity with ADP, activity with GTP is 8.1% compared to the activity with ADP, no activity with diphosphate, phosphoenolpyruvate or polyphosphate | Methanocaldococcus jannaschii | AMP + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.146 | ADP + D-fructose 6-phosphate | key enzyme of the glycolytic pathway | Methanocaldococcus jannaschii DSM 2661 | AMP + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.146 | ADP + D-fructose 6-phosphate | activity with GDP is 1.4% compared to the activity with ADP, activity with ATP is 0.3% compared to the activity with ADP, activity with GTP is 8.1% compared to the activity with ADP, no activity with diphosphate, phosphoenolpyruvate or polyphosphate | Methanocaldococcus jannaschii DSM 2661 | AMP + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.146 | D-fructose 6-phosphate + ADP | ADP can be replaced by acetylphosphate | Methanocaldococcus jannaschii | D-fructose 1,6-bisphosphate + AMP | - |
? | |
2.7.1.146 | D-fructose 6-phosphate + ADP | ADP can be replaced by acetylphosphate | Methanocaldococcus jannaschii | D-fructose 1,6-bisphosphate + AMP | - |
ir |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.146 | monomer | 1 * 53362, calculated from sequence | Methanocaldococcus jannaschii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.146 | ADP-dependent PFK | - |
Methanocaldococcus jannaschii |
2.7.1.146 | ADP-Pfk | - |
Methanocaldococcus jannaschii |
2.7.1.146 | MJ1604 | MJ1604 | Methanocaldococcus jannaschii |
2.7.1.146 | pfkC | - |
Methanocaldococcus jannaschii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.146 | 50 | - |
assay at | Methanocaldococcus jannaschii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.146 | 6.5 | - |
assay at | Methanocaldococcus jannaschii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.1.146 | physiological function | key enzyme of the modified Embden-Meyerhof pathway of heterotrophic and chemolithoautotrophic archaea | Methanocaldococcus jannaschii |