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Literature summary extracted from

  • Harrison, G.; Curle, C.; Laishley, E.J.
    Purification and characterization of an inducible dissimilatory type sulfite reductase from Clostridium pasteurianum (1984), Arch. Microbiol., 138, 72-78.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.99.5 KCN 1 mM, complete inhibition Clostridium pasteurianum
1.8.99.5 additional information not inhibitory: iodoacetate Clostridium pasteurianum
1.8.99.5 NaN3 1 mM, 18% inhibition Clostridium pasteurianum
1.8.99.5 Tris/HCl Tris buffer has an inhibitory effect. Approximately 60% less activity than with phosphate buffer is obtained at pH 7.5 Clostridium pasteurianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.99.5 0.35
-
 sulfite pH 7.5, 37°C Clostridium pasteurianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.99.5 13400
-
 2 * 13400 plus 2 * 28400, SDS-PAGE Clostridium pasteurianum
1.8.99.5 28400
-
 2 * 13400 plus 2 * 28400, SDS-PAGE Clostridium pasteurianum
1.8.99.5 83600
-
 PAGE Clostridium pasteurianum

Organism

EC Number Organism UniProt Comment Textmining
1.8.99.5 Clostridium pasteurianum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.99.5 hydroxylamine + a [DsrC protein]-dithiol + 2 reduced acceptor + 2 H+
-
 Clostridium pasteurianum ? + a [DsrC protein]-disulfide + 2 acceptor + 3 H2O 50% of the activity with sulfite ?
1.8.99.5 additional information no substrates: S3O62-, S2O32-. Methyl viologen is as effective as ferredoxin in coupling the sulfite reductase with hydrogenase while benzyl viologen is only 16% as effective as ferredoxin. No activity is observed with NAD, NADP, FAD or FMN. Varying the substrate concentration [SO 2-] from 1 to 2.5 micromol affects the stoichiometry of the enzyme reaction by alteration of the ratio of H2 uptake to S2- formed from 2.5:1 to 3.1:1 Clostridium pasteurianum ?
-
 ?
1.8.99.5 nitrite + a [DsrC protein]-dithiol + 2 reduced acceptor
-
 Clostridium pasteurianum ? + a [DsrC protein]-disulfide + 2 acceptor + 2 H2O 25% of the activity with sulfite ?
1.8.99.5 SeO32- + a [DsrC protein]-dithiol + 2 reduced methyl viologen + 2 H+
-
 Clostridium pasteurianum HSe- + a [DsrC protein]-disulfide + 2 oxidized methyl viologen + 3 H2O 40% of the activity with sulfite ?
1.8.99.5 sulfite + a [DsrC protein]-dithiol + 2 reduced ferredoxin + 2 H+
-
 Clostridium pasteurianum hydrogen sulfide + a [DsrC protein]-disulfide + 2 oxidized ferredoxin + 3 H2O overall reaction ?

Subunits

EC Number Subunits Comment Organism
1.8.99.5 heterotetramer 2 * 13400 plus 2 * 28400, SDS-PAGE Clostridium pasteurianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.99.5 7.5
-
 phosphate buffer Clostridium pasteurianum

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.99.5 additional information no activity is observed with NAD, NADP, FAD or FMN Clostridium pasteurianum