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Literature summary extracted from

  • Hultquist, D.E.
    Methemoglobin reduction system of erythrocytes (1978), Methods Enzymol., 52, 463-473.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.6.2.2 enzyme from erythrocyte loses its activity in the absence of EDTA after 5 d at 4°C Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.2 K+ high ionic strength reduction of cytochrome b5 or dichlorphenolindophenol Homo sapiens
1.6.2.2 Tris reduction of cytochrome b5 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.00016
-
 NADH enzyme from erythrocyte Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.2 cytosol
-
 Homo sapiens 5829
-
1.6.2.2 endoplasmic reticulum
-
 Homo sapiens 5783
-

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.2
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.2 erythrocyte soluble form Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
1.6.2.2 -20°C, pH 7.5-8.1, 0.5 mM EDTA, protein concentration above 0.02 mg/ml Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 2 ferricytochrome b5 + NADH specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol Homo sapiens 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 2 ferricytochrome b5 + NADH poor electron acceptors: methylene blue, ferricytochrome c, O2, oxidized glutathione, methemoglobin Homo sapiens 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 NADH + ferricytochrome b5
-
 Homo sapiens NAD+ + H+ + ferrocytochrome b5
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.2 21.3
-
 ferricytochrome b5
-
 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.6.2.2 7.5 8.1
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 FAD
-
 Homo sapiens
1.6.2.2 NADH
-
 Homo sapiens