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Literature summary extracted from

  • Bick, J.A.; Dennis, J.J.; Zylstra, G.J.; Nowack, J.; Leustek, T.
    Identification of a new class of 5'-adenylylsulfate (APS) reductases from sulfate-assimilating bacteria (2000), J. Bacteriol., 182, 135-142.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.4.10 additional information not activated by sodium sulfate Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.10 expression in Escherichia coli Pseudomonas putida
1.8.99.2
-
 Pseudomonas aeruginosa
1.8.99.2
-
 Burkholderia cepacia

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.4.10 5'-AMP competitive vs. adenosine-5'-phosphosulfate Pseudomonas aeruginosa
1.8.4.10 adenosine-5'-phosphosulfate
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.10 0.00175
-
 adenosine-5'-phosphosulfate
-
 Pseudomonas aeruginosa
1.8.4.10 0.0196
-
 thioredoxin
-
 Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.10 5'-adenylyl sulfate + thioredoxin Pseudomonas aeruginosa no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin Pseudomonas putida involved in assimilatory sulfate reduction AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin Mycobacterium sp. involved in assimilatory sulfate reduction AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin Ralstonia pickettii involved in assimilatory sulfate reduction AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin Rhizobium tropici involved in assimilatory sulfate reduction AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin Burkholderia cepacia involved in assimilatory sulfate reduction AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin Pseudomonas aeruginosa involved in assimilatory sulfate reduction AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin Burkholderia cepacia DBO1 / ATCC 29424 involved in assimilatory sulfate reduction AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor Escherichia coli
-
 AMP + sulfite + acceptor
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor Bacillus subtilis assimilatory sulfate reduction AMP + sulfite + acceptor
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor Pseudomonas aeruginosa assimilatory sulfate reduction AMP + sulfite + acceptor
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor Pseudomonas putida assimilatory sulfate reduction AMP + sulfite + acceptor
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor Mycobacterium sp. assimilatory sulfate reduction AMP + sulfite + acceptor
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor Burkholderia cepacia assimilatory sulfate reduction AMP + sulfite + acceptor
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor Ralstonia pickettii assimilatory sulfate reduction AMP + sulfite + acceptor
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor Rhizobium tropici assimilatory sulfate reduction AMP + sulfite + acceptor
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor Burkholderia cepacia DBO1 / ATCC 29424 assimilatory sulfate reduction AMP + sulfite + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.10 Burkholderia cepacia Q9RFS6
-
-
1.8.4.10 Burkholderia cepacia DBO1 / ATCC 29424 Q9RFS6
-
-
1.8.4.10 Mycobacterium sp.
-
-
-
1.8.4.10 Pseudomonas aeruginosa O05927
-
-
1.8.4.10 Pseudomonas putida
-
-
-
1.8.4.10 Ralstonia pickettii
-
-
-
1.8.4.10 Rhizobium tropici
-
-
-
1.8.99.2 Bacillus subtilis
-
-
-
1.8.99.2 Burkholderia cepacia
-
 DBO1
-
1.8.99.2 Burkholderia cepacia DBO1 / ATCC 29424
-
 DBO1
-
1.8.99.2 Escherichia coli
-
-
-
1.8.99.2 Mycobacterium sp.
-
-
-
1.8.99.2 Pseudomonas aeruginosa
-
-
-
1.8.99.2 Pseudomonas putida
-
-
-
1.8.99.2 Ralstonia pickettii
-
-
-
1.8.99.2 Rhizobium tropici
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.99.2
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.99.2 5.8
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.10 5'-adenylyl sulfate + thioredoxin
-
 Pseudomonas putida AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin
-
 Mycobacterium sp. AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin
-
 Ralstonia pickettii AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin
-
 Rhizobium tropici AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin
-
 Burkholderia cepacia AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS Pseudomonas aeruginosa AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin involved in assimilatory sulfate reduction Pseudomonas putida AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin involved in assimilatory sulfate reduction Mycobacterium sp. AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin involved in assimilatory sulfate reduction Ralstonia pickettii AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin involved in assimilatory sulfate reduction Rhizobium tropici AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin involved in assimilatory sulfate reduction Burkholderia cepacia AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin involved in assimilatory sulfate reduction Pseudomonas aeruginosa AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin
-
 Burkholderia cepacia DBO1 / ATCC 29424 AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin involved in assimilatory sulfate reduction Burkholderia cepacia DBO1 / ATCC 29424 AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor
-
 Escherichia coli AMP + sulfite + acceptor
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor assimilatory sulfate reduction Bacillus subtilis AMP + sulfite + acceptor
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor assimilatory sulfate reduction Pseudomonas aeruginosa AMP + sulfite + acceptor
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor assimilatory sulfate reduction Pseudomonas putida AMP + sulfite + acceptor
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor assimilatory sulfate reduction Mycobacterium sp. AMP + sulfite + acceptor
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor assimilatory sulfate reduction Burkholderia cepacia AMP + sulfite + acceptor
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor assimilatory sulfate reduction Ralstonia pickettii AMP + sulfite + acceptor
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor assimilatory sulfate reduction Rhizobium tropici AMP + sulfite + acceptor
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor assimilatory sulfate reduction Burkholderia cepacia DBO1 / ATCC 29424 AMP + sulfite + acceptor
-
 ?
1.8.99.2 adenylylsulfate + thioredoxin
-
 Pseudomonas aeruginosa AMP + sulfite + ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.4.10 8.5
-
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.10 thioredoxin
-
 Pseudomonas putida
1.8.4.10 thioredoxin
-
 Mycobacterium sp.
1.8.4.10 thioredoxin
-
 Ralstonia pickettii
1.8.4.10 thioredoxin
-
 Rhizobium tropici
1.8.4.10 thioredoxin
-
 Burkholderia cepacia
1.8.4.10 thioredoxin 5fold lower activity with dithiothreithol, 70fold lower activity with E. coli Grx1 or dithionite, 580fold lower activity with lipoic acid, no activity with ferredoxin Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.4.10 1
-
 adenosine-5'-phosphosulfate
-
 Pseudomonas aeruginosa