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Literature summary extracted from

  • Khanna, S.; Nicholas, D.J.D.
    Substrate phosphorylation in Chlorobium vibrioforme f. sp. thiosulfatophilum (1983), J. Gen. Microbiol., 129, 1365-1370.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.7.5 very labile in crude extract, partially stabilized by addition of EDTA and 2-mercaptoethanol Prosthecochloris vibrioformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.99.2 AMP above 2 mM Prosthecochloris vibrioformis
1.8.99.2 iodoacetamide 5 mM, 54% inhibition Prosthecochloris vibrioformis
1.8.99.2 NEM 5 mM, 49% inhibition Prosthecochloris vibrioformis
1.8.99.2 PCMB 1 mM, 30% inhibition Prosthecochloris vibrioformis
2.7.7.5 iodoacetamide
-
 Prosthecochloris vibrioformis
2.7.7.5 N-ethylmaleimide very potent inhibitor, partially reversible by thiol-containing reagents Prosthecochloris vibrioformis
2.7.7.5 p-chloromercuribenzoate very potent inhibitor, partially reversible by thiol-containing reagents Prosthecochloris vibrioformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.99.2 0.13
-
 AMP
-
 Prosthecochloris vibrioformis
1.8.99.2 0.17
-
 sulfite
-
 Prosthecochloris vibrioformis
1.8.99.2 0.25
-
 adenylyl sulfate
-
 Prosthecochloris vibrioformis
1.8.99.2 4.2
-
 ferricyanide
-
 Prosthecochloris vibrioformis
2.7.7.5 0.25
-
 adenosine 5'-phosphosulfate
-
 Prosthecochloris vibrioformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.99.2 Iron contains 4-6 mol of non-haem iron per mol of enzyme Prosthecochloris vibrioformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.99.2 180000
-
 gel filtration Prosthecochloris vibrioformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.5 phosphate + adenosine 5'-phosphosulfate Prosthecochloris vibrioformis
-
 ADP + SO42-
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.99.2 Prosthecochloris vibrioformis
-
 f. sp. thiosulfatophilum
-
2.7.7.5 Prosthecochloris vibrioformis
-
 f. sp. thiosulfatophilum, strain NCIB 8346
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.99.2
-
 Prosthecochloris vibrioformis
2.7.7.5
-
 Prosthecochloris vibrioformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.99.2 13.15
-
-
 Prosthecochloris vibrioformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.99.2 AMP + sulfite + ferricyanide
-
 Prosthecochloris vibrioformis adenylyl sulfate + ferrocyanide
-
 ?
1.8.99.2 GMP + sulfite + ferricyanide 54% of the activity with AMP Prosthecochloris vibrioformis guanylyl sulfate + ferrocyanide
-
 ?
2.7.7.5 phosphate + adenosine 5'-phosphosulfate
-
 Prosthecochloris vibrioformis ADP + SO42-
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.99.2 8
-
-
 Prosthecochloris vibrioformis

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.99.2 FAD
-
 Prosthecochloris vibrioformis