Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Koga, D.; Hoshika, H.; Matsushita, M.; Tanaka, A.; Ide, A.; Kono, M.
    Purification and characterization of beta-N-acetylhexosaminidase from the liver of a prawn, Penaeus japonicus (1996), Biosci. Biotechnol. Biochem., 60, 194-199.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 Hg2+ concentration of 10 mM causes 39% inhibition Penaeus japonicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.044
-
 (N-acetyl-beta-D-glucosamine)4
-
 Penaeus japonicus
3.2.1.52 0.045
-
 (N-acetyl-beta-D-glucosamine)5
-
 Penaeus japonicus
3.2.1.52 0.047
-
 (N-acetyl-beta-D-glucosamine)6
-
 Penaeus japonicus
3.2.1.52 0.055
-
 (N-acetyl-beta-D-glucosamine)3
-
 Penaeus japonicus
3.2.1.52 0.117
-
 (N-acetyl-beta-D-glucosamine)2
-
 Penaeus japonicus
3.2.1.52 0.137
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Penaeus japonicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 64000
-
 SDS-PAGE Penaeus japonicus
3.2.1.52 110000
-
 gel filtration Penaeus japonicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Penaeus japonicus
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Penaeus japonicus
-
 prawn
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 699fold Penaeus japonicus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.52 (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose exo-type reaction Penaeus japonicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 liver
-
 Penaeus japonicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 123000
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Penaeus japonicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 (N-acetyl-beta-D-glucosamine)2 + H2O
-
 Penaeus japonicus 2 N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 (N-acetyl-beta-D-glucosamine)3 + H2O
-
 Penaeus japonicus (N-acetyl-beta-D-glucosamine)2 + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 (N-acetyl-beta-D-glucosamine)4 + H2O
-
 Penaeus japonicus (N-acetyl-beta-D-glucosamine)3 + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 (N-acetyl-beta-D-glucosamine)5 + H2O
-
 Penaeus japonicus (N-acetyl-beta-D-glucosamine)4 + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 (N-acetyl-beta-D-glucosamine)6 + H2O
-
 Penaeus japonicus (N-acetyl-beta-D-glucosamine)5 + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Penaeus japonicus 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
 ?
3.2.1.52 chitin oligosaccharides + H2O (N-acetyl-D-glucosamine)2-6 Penaeus japonicus N-acetyl-D-glucosamine
-
 ?
3.2.1.52 N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Penaeus japonicus ?
-
 ?
3.2.1.52 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Penaeus japonicus ?
-
 ?
3.2.1.52 N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Penaeus japonicus 2 N,N-diacetylchitobiose
-
 ?
3.2.1.52 N,N',N''-triacetylchitotriose + H2O
-
 Penaeus japonicus N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
 ?
3.2.1.52 N,N'-diacetylchitobiose + H2O
-
 Penaeus japonicus deacetylated chitooligosaccharides + acetate
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Penaeus japonicus N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Penaeus japonicus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 dimer 2 * 64000, SDS-PAGE Penaeus japonicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 50
-
-
 Penaeus japonicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 55
-
 stable below Penaeus japonicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.52 8.3
-
 (N-acetyl-beta-D-glucosamine)6
-
 Penaeus japonicus
3.2.1.52 14.7
-
 (N-acetyl-beta-D-glucosamine)5
-
 Penaeus japonicus
3.2.1.52 30.1
-
 (N-acetyl-beta-D-glucosamine)4
-
 Penaeus japonicus
3.2.1.52 96.4
-
 (N-acetyl-beta-D-glucosamine)3
-
 Penaeus japonicus
3.2.1.52 298
-
 (N-acetyl-beta-D-glucosamine)2
-
 Penaeus japonicus
3.2.1.52 598
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Penaeus japonicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 5 5.5
-
 Penaeus japonicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 4 11 stable Penaeus japonicus