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Literature summary extracted from
- Purification and properties of cellulase from Phaseolus vulgaris (1974), Phytochemistry, 13, 1359-1366.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.4 | Ag+ | 0.1 mM, strong inhibition | Phaseolus vulgaris |  |
| 3.2.1.4 | cellobiose | - |
Phaseolus vulgaris | |
| 3.2.1.4 | Hg2+ | 1 mM, strong inhibition | Phaseolus vulgaris | |
| 3.2.1.4 | NEM | 70% inhibition of cellulase 4.5 at 10 mM, no effect on cellulase 4.8 | Phaseolus vulgaris | |
| 3.2.1.4 | PCMB | 0.1 mM, 90% loss of activity of cellulase 4.5, 50% loss of activity of cellulase 4.8 | Phaseolus vulgaris | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 70000 | - |
gel filtration | Phaseolus vulgaris |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.4 | Phaseolus vulgaris | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.4 | cellulase 4.5 and cellulase 4.8 | Phaseolus vulgaris |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.4 | cotyledon | - |
Phaseolus vulgaris | - |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 35 | 43 | cellulase 4.8, hydrolysis of carboxymethylcellulose | Phaseolus vulgaris |
| 3.2.1.4 | 45 | 60 | cellulase 4.5, hydrolysis of carboxymethylcellulose | Phaseolus vulgaris |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 36 | - |
30 min, 50% loss of activity of cellulase 4.8, 25% loss of activity of cellulase 4.5 | Phaseolus vulgaris |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 4.8 | 5.6 | cellulase 4.8 | Phaseolus vulgaris |
| 3.2.1.4 | 5.7 | 6.2 | cellulase 4.5 | Phaseolus vulgaris |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 6 | 9 | cellulase 4.5, stable | Phaseolus vulgaris |
| 3.2.1.4 | 6.5 | 8 | cellulase 4.8, stable | Phaseolus vulgaris |
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