EC Number | General Stability | Organism |
---|---|---|
3.2.1.1 | free Ca2+, slight stabilization | Pyrococcus furiosus |
3.2.1.1 | guanidine HCl, 1 M, 27% loss of activity, significant decrease in activity at 2 M | Pyrococcus furiosus |
3.2.1.1 | moderate protease susceptibility | Pyrococcus furiosus |
3.2.1.1 | urea, 1 M, 13% loss of activity, significant decrease in activity at 2 M | Pyrococcus furiosus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.1 | Co2+ | - |
Pyrococcus furiosus | |
3.2.1.1 | Cr2+ | - |
Pyrococcus furiosus | |
3.2.1.1 | Cu2+ | - |
Pyrococcus furiosus | |
3.2.1.1 | Fe2+ | - |
Pyrococcus furiosus | |
3.2.1.1 | Mg2+ | - |
Pyrococcus furiosus | |
3.2.1.1 | Zn2+ | - |
Pyrococcus furiosus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.1 | additional information | - |
additional information | - |
Pyrococcus furiosus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.1 | intracellular | - |
Pyrococcus furiosus | 5622 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 66000 | - |
2 * 66000, intracellular enzyme, electrophoresis in presence of 8 M urea | Pyrococcus furiosus |
3.2.1.1 | 129000 | - |
non-denaturing PAGE | Pyrococcus furiosus |
3.2.1.1 | 130500 | - |
equilibrium ultracentrifugation | Pyrococcus furiosus |
3.2.1.1 | 157000 | - |
gel filtration | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.1 | Pyrococcus furiosus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.1 | - |
Pyrococcus furiosus |
EC Number | Renatured (Comment) | Organism |
---|---|---|
3.2.1.1 | heat denaturation is irreversible | Pyrococcus furiosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.1 | maltoheptaose + H2O | - |
Pyrococcus furiosus | additional information | - |
? | |
3.2.1.1 | maltohexaose + H2O | - |
Pyrococcus furiosus | additional information | - |
? | |
3.2.1.1 | maltotriose | the final optimum also mirrors the reverse reaction | Pyrococcus furiosus | maltose + glucose + maltotetraose + maltopentaose + maltohexaose | - |
r | |
3.2.1.1 | maltotriose + H2O | - |
Pyrococcus furiosus | maltose + D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.1 | dimer | 2 * 66000, intracellular enzyme, electrophoresis in presence of 8 M urea | Pyrococcus furiosus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 100 | - |
- |
Pyrococcus furiosus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 70 | 115 | 70°C: about 30% of maximal activity, 115°C: about 75% of maximal activity | Pyrococcus furiosus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | additional information | - |
the process of heat denaturation is complex and includes at least three stages, indicating that the protein structure consists of three domains, heat denaturation is irreversible | Pyrococcus furiosus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 6.5 | 7.5 | - |
Pyrococcus furiosus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 6 | 8 | pH 6.0: about 50% of maximal activity, pH 8.0: about 60% of maximal activity | Pyrococcus furiosus |