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Literature summary extracted from
- Purification and some properties of an extracellular alpha-amylase from Bacteroides amylophilus (1977), J. Bacteriol., 129, 1537-1544.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | alpha-D-phenylglucoside | - |
Ruminobacter amylophilus |  |
| 3.2.1.1 | cyclomaltoheptaose | - |
Ruminobacter amylophilus | |
| 3.2.1.1 | cyclomaltohexaose | - |
Ruminobacter amylophilus | |
| 3.2.1.1 | EDTA | 0.01 M, 58% loss of activity | Ruminobacter amylophilus | |
| 3.2.1.1 | Hg2+ | 5 mM, 85% inhibition | Ruminobacter amylophilus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.1 | additional information | - |
additional information | - |
Ruminobacter amylophilus | |
| 3.2.1.1 | 16.7 | - |
Dextrin | micromol of D-glucosidic bond per liter | Ruminobacter amylophilus | |
| 3.2.1.1 | 18.2 | - |
amylose | micromol of D-glucosidic bond per liter | Ruminobacter amylophilus | |
| 3.2.1.1 | 18.2 | - |
amylopectin | micromol of D-glucosidic bond per liter | Ruminobacter amylophilus | |
| 3.2.1.1 | 18.7 | - |
starch | micromol of D-glucosidic bond per L | Ruminobacter amylophilus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.1 | extracellular | - |
Ruminobacter amylophilus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | Ba2+ | 5 mM, slight activation | Ruminobacter amylophilus | |
| 3.2.1.1 | Ca2+ | 5 mM, about 70% activation | Ruminobacter amylophilus | |
| 3.2.1.1 | Co2+ | 5 mM, about 70% activation | Ruminobacter amylophilus | |
| 3.2.1.1 | Cu2+ | 5 mM, slight activation | Ruminobacter amylophilus | |
| 3.2.1.1 | Fe2+ | 5 mM, slight activation | Ruminobacter amylophilus | |
| 3.2.1.1 | K+ | 5 mM, slight activation | Ruminobacter amylophilus | |
| 3.2.1.1 | Li+ | 5 mM, slight activation | Ruminobacter amylophilus | |
| 3.2.1.1 | Mg2+ | 5 mM, slight activation | Ruminobacter amylophilus | |
| 3.2.1.1 | Na+ | 5 mM, slight activation | Ruminobacter amylophilus | |
| 3.2.1.1 | Pb2+ | 5 mM, slight activation | Ruminobacter amylophilus | |
| 3.2.1.1 | Zn2+ | 5 mM, slight activation | Ruminobacter amylophilus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 92000 | - |
x * 92000, SDS-PAGE | Ruminobacter amylophilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Ruminobacter amylophilus | - |
- |
- |
| 3.2.1.1 | Ruminobacter amylophilus 70 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | - |
Ruminobacter amylophilus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 122 | - |
- |
Ruminobacter amylophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | amylopectin + H2O | 92% of the activity with amylose | Ruminobacter amylophilus | additional information | - |
? | |
| 3.2.1.1 | amylopectin + H2O | 92% of the activity with amylose | Ruminobacter amylophilus 70 | additional information | - |
? | |
| 3.2.1.1 | amylose + H2O | - |
Ruminobacter amylophilus | additional information | - |
? | |
| 3.2.1.1 | amylose + H2O | - |
Ruminobacter amylophilus 70 | additional information | - |
? | |
| 3.2.1.1 | dextrin + H2O | - |
Ruminobacter amylophilus | ? | - |
? | |
| 3.2.1.1 | dextrin + H2O | - |
Ruminobacter amylophilus 70 | ? | - |
? | |
| 3.2.1.1 | maltodextrin + H2O | 60% of the activity with amylose | Ruminobacter amylophilus | ? | - |
? | |
| 3.2.1.1 | maltotetraose + H2O | - |
Ruminobacter amylophilus | additional information | maltose + maltose | ? | |
| 3.2.1.1 | maltotetraose + H2O | - |
Ruminobacter amylophilus 70 | additional information | maltose + maltose | ? | |
| 3.2.1.1 | starch + H2O | soluble starch | Ruminobacter amylophilus | additional information | products in the late stage of reaction: maltose + maltotriose with less amounts of maltotetraose and maltopentaose and traces of glucose | ? | |
| 3.2.1.1 | starch + H2O | soluble starch | Ruminobacter amylophilus 70 | additional information | products in the late stage of reaction: maltose + maltotriose with less amounts of maltotetraose and maltopentaose and traces of glucose | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | ? | x * 92000, SDS-PAGE | Ruminobacter amylophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 43 | - |
- |
Ruminobacter amylophilus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 25 | 50 | 25°C, about 50% of maximal activity, 50°C: about 85% of maximal activity | Ruminobacter amylophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 6.3 | - |
- |
Ruminobacter amylophilus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 5 | 8 | about 45% of maximal activity at pH 5.0 and at pH 8.0 | Ruminobacter amylophilus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 5.8 | 7.5 | 25°C, 0.067 M phosphate buffer, enzyme retains over 93% of its activity | Ruminobacter amylophilus |
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