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Literature summary extracted from
- Purification and characterization of an alpha-glucosidase from a hyperthermophilic archaebacterium, Pyrococcus furiosus, exhibiting a temperature optimum of 105 to 115¦C (1990), J. Bacteriol., 172, 3654-3660.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.20 | guanidine hydrochloride | - |
Pyrococcus furiosus |  |
| 3.2.1.20 | maltose | inhibits hydrolysis of p-nitrophenyl-alpha-D-glucopyranoside | Pyrococcus furiosus | |
| 3.2.1.20 | SDS | significant but not complete loss of activity | Pyrococcus furiosus | |
| 3.2.1.20 | Urea | - |
Pyrococcus furiosus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.20 | 0.26 | - |
p-nitrophenyl-alpha-D-glucopyranoside | - |
Pyrococcus furiosus | |
| 3.2.1.20 | 0.77 | - |
methyl-alpha-D-glucopyranoside | - |
Pyrococcus furiosus | |
| 3.2.1.20 | 5.2 | - |
maltose | - |
Pyrococcus furiosus | |
| 3.2.1.20 | 8.2 | - |
isomaltose | - |
Pyrococcus furiosus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.20 | cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.20 | Pyrococcus furiosus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.20 | - |
Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | additional information | - |
- |
Pyrococcus furiosus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.20 | isomaltose + H2O | - |
Pyrococcus furiosus | 2 alpha-D-glucose | - |
? | |
| 3.2.1.20 | maltose + H2O | - |
Pyrococcus furiosus | alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.20 | methyl-alpha-glucoside + H2O | - |
Pyrococcus furiosus | methanol + alpha-D-glucose | - |
? | |
| 3.2.1.20 | p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + D-glucose | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 105 | 115 | - |
Pyrococcus furiosus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 95 | 115 | 95°C: about 50% of maximal activity, 105-115°C: optimum | Pyrococcus furiosus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 98 | - |
half-life: 48 h | Pyrococcus furiosus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 5 | 6 | - |
Pyrococcus furiosus |
| 3.2.1.20 | 5.5 | - |
- |
Pyrococcus furiosus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 4.5 | 7.3 | pH 4.5: about 80% of maximal activity, pH 7.3: about 50% of maximal activity | Pyrococcus furiosus |
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