Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Berthelot, K.; Delmotte, F.M.
    Purification and characterization of an alpha-glucosidase from Rhizobium sp. (Robinia pseudoacacia L.) strain USDA 4280 (1999), Appl. Environ. Microbiol., 65, 2907-2911.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.20 NH4+ activates Rhizobium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 acetosyringone
-
 Rhizobium sp.
3.2.1.20 Ag+
-
 Rhizobium sp.
3.2.1.20 Ca2+ 10 mM, slight inhibition Rhizobium sp.
3.2.1.20 Co2+ 10 mM, slight inhibition Rhizobium sp.
3.2.1.20 Cu2+
-
 Rhizobium sp.
3.2.1.20 dithiothreitol 50 mM Rhizobium sp.
3.2.1.20 Fe2+
-
 Rhizobium sp.
3.2.1.20 Fe3+
-
 Rhizobium sp.
3.2.1.20 Hg2+
-
 Rhizobium sp.
3.2.1.20 Li+ 10 mM, slight inhibition Rhizobium sp.
3.2.1.20 Mg2+ 10 mM, slight inhibition Rhizobium sp.
3.2.1.20 Mn2+ 10 mM, slight inhibition Rhizobium sp.
3.2.1.20 SDS 0.1% Rhizobium sp.
3.2.1.20 Tris
-
 Rhizobium sp.
3.2.1.20 Urea 4 M Rhizobium sp.
3.2.1.20 Zn2+ 10 mM, slight inhibition Rhizobium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 0.037
-
 4-nitrophenyl alpha-D-glucoside
-
 Rhizobium sp.
3.2.1.20 0.141
-
 4-methylumbelliferyl-alpha-glucoside
-
 Rhizobium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.20 K+ stimulates Rhizobium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 59000
-
 gel filtration Rhizobium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Rhizobium sp.
-
-
-
3.2.1.20 Rhizobium sp. USDA 4280
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20
-
 Rhizobium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.20 seed
-
 Rhizobium sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 5.72
-
-
 Rhizobium sp.

Storage Stability

EC Number Storage Stability Organism
3.2.1.20 -20°C, stable for months Rhizobium sp.
3.2.1.20 4°C, stable for several weeks Rhizobium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-methylumbelliferyl-alpha-glucoside + H2O
-
 Rhizobium sp. 4-methylumbelliferone + alpha-D-glucose
-
 ?
3.2.1.20 4-methylumbelliferyl-alpha-glucoside + H2O
-
 Rhizobium sp. USDA 4280 4-methylumbelliferone + alpha-D-glucose
-
 ?
3.2.1.20 4-nitrophenyl alpha-D-glucoside
-
 Rhizobium sp. 4-nitrophenol + alpha-D-glucose
-
 ?
3.2.1.20 4-nitrophenyl alpha-D-glucoside
-
 Rhizobium sp. USDA 4280 4-nitrophenol + alpha-D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Rhizobium sp. alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Rhizobium sp. USDA 4280 alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 sucrose + H2O
-
 Rhizobium sp. D-fructose + alpha-D-glucose
-
 ?
3.2.1.20 sucrose + H2O
-
 Rhizobium sp. USDA 4280 D-fructose + alpha-D-glucose
-
 ?
3.2.1.20 trehalose + H2O
-
 Rhizobium sp. alpha-D-glucose
-
 ?
3.2.1.20 trehalose + H2O
-
 Rhizobium sp. USDA 4280 alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 monomer 1 * 59000, SDS-PAGE und reducing and under nonreducing conditions Rhizobium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 35
-
-
 Rhizobium sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.20 15 50 15°C: about 35% of maximal activity, 50°C: about 25% of maximal activity Rhizobium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 6 6.5
-
 Rhizobium sp.