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Literature summary extracted from

  • Cane, D.E.; Chow, C.; Lillo, A.; Kang, I.
    Molecular cloning, expression and characterization of the first three genes in the mevalonate-independent isoprenoid pathway in Streptomyces coelicolor (2001), Bioorg. Med. Chem., 9, 1467-1477.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.60 expressed in Escherichia coli BL21(DE3)-RP Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.60 0.032
-
 2-C-methyl-D-erythritol-4-phosphate
-
 Escherichia coli
2.7.7.60 1.45
-
 CTP
-
 Streptomyces coelicolor
2.7.7.60 3.26
-
 2-C-methyl-D-erythritol-4-phosphate
-
 Streptomyces coelicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.60 27712
-
 x * 27712, ES-MS Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.60 CTP + 2-C-methyl-D-erythritol-4-phosphate Escherichia coli part of mevalonate-indpendent pathway for isoprenoid biosynthesis diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol-4-phosphate Streptomyces coelicolor part of mevalonate-indpendent pathway for isoprenoid biosynthesis diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol-4-phosphate Streptomyces coelicolor CH999 part of mevalonate-indpendent pathway for isoprenoid biosynthesis diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.60 Escherichia coli
-
-
-
2.7.7.60 Streptomyces coelicolor
-
 CH999
-
2.7.7.60 Streptomyces coelicolor CH999
-
 CH999
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.60 Q-Sepharose, Phenyl-Sepharose, Superdex HiLoad Streptomyces coelicolor

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.60 0.00335
-
 assay with D-1-deoxyxylulose-5-phosphate or 2-C-methyl-D-erythritol-4-phosphate Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.60 CTP + 2-C-methyl-D-erythritol 4-phosphate
-
 Escherichia coli diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol 4-phosphate
-
 Streptomyces coelicolor diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol 4-phosphate
-
 Streptomyces coelicolor CH999 diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol-4-phosphate part of mevalonate-indpendent pathway for isoprenoid biosynthesis Escherichia coli diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol-4-phosphate part of mevalonate-indpendent pathway for isoprenoid biosynthesis Streptomyces coelicolor diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol-4-phosphate part of mevalonate-indpendent pathway for isoprenoid biosynthesis Streptomyces coelicolor CH999 diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.60 ? x * 27712, ES-MS Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.60 30
-
-
 Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.60 1.4
-
 2-C-methyl-D-erythritol-4-phosphate
-
 Streptomyces coelicolor
2.7.7.60 6
-
 CTP
-
 Streptomyces coelicolor
2.7.7.60 16.8
-
 2-C-methyl-D-erythritol-4-phosphate
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.60 7.9
-
-
 Streptomyces coelicolor