Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Barber, M.J.; Notton, B.A.
    Spinach nitrate reductase (1990), Plant Physiol., 93, 537-540.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.1 0.006
-
 ferricytochrome c ionic strength: 50 mM Spinacia oleracea
1.7.1.1 0.006
-
 NADH ionic strength: 200 mM Spinacia oleracea
1.7.1.1 0.007
-
 NADH ionic strength: 50 mM Spinacia oleracea
1.7.1.1 0.013
-
 nitrate at ionic strength of 50 mM Spinacia oleracea
1.7.1.1 0.018
-
 nitrate at ionic strength of 200 mM Spinacia oleracea
1.7.1.1 0.021
-
 ferricyanide ionic strength: 50 mM Spinacia oleracea
1.7.1.1 0.029
-
 ferricytochrome c ionic strength: 200 mM Spinacia oleracea
1.7.1.1 0.035
-
 ferricyanide ionic strength: 200 mM Spinacia oleracea
1.7.1.1 0.053
-
 oxidized 2,6-dichlorophenolindophenol ionic strength: 50 mM Spinacia oleracea
1.7.1.1 0.056
-
 oxidized 2,6-dichlorophenolindophenol ionic strength: 200 mM Spinacia oleracea
1.7.1.1 0.056
-
 flavin hydroquinone ionic strength: 50 mM or 200 mM Spinacia oleracea
1.7.1.1 0.221
-
 ClO3- ionic strength: 50 mM Spinacia oleracea
1.7.1.1 0.739
-
 BrO3- ionic strength: 50 mM Spinacia oleracea
1.7.1.1 10.75
-
 IO3- ionic strength: 50 mM Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.1 Spinacia oleracea
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.1.1 root
-
 Spinacia oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.1 2 ferricyanide + NADH
-
 Spinacia oleracea 2 ferrocyanide + NAD+ + H+
-
 ?
1.7.1.1 2 ferricytochrome c + NADH
-
 Spinacia oleracea 2 ferrocytochrome c + NAD+ + H+
-
 ?
1.7.1.1 flavin hydroquinone + nitrate
-
 Spinacia oleracea ? + nitrite
-
 ?
1.7.1.1 NADH + BrO3-
-
 Spinacia oleracea NAD+ + ?
-
 ?
1.7.1.1 NADH + ClO3-
-
 Spinacia oleracea NAD+ + ?
-
 ?
1.7.1.1 NADH + IO3-
-
 Spinacia oleracea NAD+ + ?
-
 ?
1.7.1.1 nitrate + NADH
-
 Spinacia oleracea nitrite + NAD+ + H2O
-
 ?
1.7.1.1 oxidized 2,6-dichlorophenolindophenol + NADH
-
 Spinacia oleracea ? + NAD+
-
 ?
1.7.1.1 oxidized 2,6-dichlorophenolindophenol + NADH + H+
-
 Spinacia oleracea reduced 2,6-dichlorophenolindophenol + NAD+
-
 r
1.7.1.1 reduced methyl viologen + nitrate
-
 Spinacia oleracea methyl viologen + nitrite
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.1 6.5
-
 NADH:ferricyanide reductase activity, NADH:dichlorophenolindophenol reductase activity and reduced methyl viologen:nitrate reductase activity Spinacia oleracea
1.7.1.1 7
-
 NADH:nitrate reductase activity Spinacia oleracea
1.7.1.1 7.3
-
 NADH:cytochrome c reductase activity Spinacia oleracea
1.7.1.1 7.5
-
 flavin hydroquinone:nitrate reductase activity Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.1 NADH
-
 Spinacia oleracea