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Literature summary extracted from

  • Bergkvist, A.; Johansson, C.; Johansson, T.; Rydström, J.; Karlsson, g.
    Interactions of the NADP(H)-binding domain III of proton-translocating transhydrogenase from Escherichia coli with NADP(H) and the NAD(H)-binding domain I studied by NMR and site-directed mutagenesis (2000), Biochemistry, 39, 12595-12605.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.1.3 expressed in Escherichia coli BL21(DE3) cells Escherichia coli
7.1.1.1 expression of domain I in Escherichia coli Rhodospirillum rubrum
7.1.1.1 expression of wild-type domain III, T393C, R425C, G430C and A432C mutant domain III in Escherichia coli Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.6.1.3 A432C the mutant shows altered NADP(H) binding and domain interaction properties Escherichia coli
1.6.1.3 G430C the mutant shows altered NADP(H) binding and domain interaction properties Escherichia coli
1.6.1.3 R425C the mutant shows almost exclusively changes in the NADP(H)-binding properties, without changing the affinity for transhydrogenase domain I from Rhodospirillum rubrum Escherichia coli
1.6.1.3 T393C the mutant shows altered NADP(H) binding and domain interaction properties Escherichia coli
7.1.1.1 A432C mutation in domain III, reverse reaction in the presence of domain I from R. rubrum, 150% higher reaction rate than wild-type domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 G430C mutation in domain III, reverse reaction in the presence of domain I from R. rubrum, 850% higher reaction rate than wild-type domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 R425C mutation in domain III, reverse reaction in the presence of domain I from R. rubrum, 425% higher reaction rate than wild-type domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 T393C mutation in domain III, reverse reaction in the presence of domain I from R. rubrum, 175% higher reaction rate than wild-type domain III/R. rubrum domain I mixture Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.1.3 NADPH + NAD+ Escherichia coli
-
 NADP+ + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.6.1.3 Escherichia coli
-
-
-
7.1.1.1 Escherichia coli
-
-
-
7.1.1.1 Rhodospirillum rubrum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.1.3
-
 Escherichia coli
7.1.1.1 recombinant domain I Rhodospirillum rubrum
7.1.1.1 recombinant wild-type, T393C, R425C, G430C and A432C mutant domain III Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.1.3 NADPH + NAD+
-
 Escherichia coli NADP+ + NADH
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.6.1.3 proton-translocating transhydrogenase domain III
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1.1.1 0.0667
-
 reduced acetylpyridine adenine dinucleotide reverse reaction of wild-type domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 0.1
-
 reduced acetylpyridine adenine dinucleotide reverse reaction of A432C mutant domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 0.117
-
 reduced acetylpyridine adenine dinucleotide reverse reaction of D393C mutant domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 0.167
-
 reduced acetylpyridine adenine dinucleotide reverse reaction of H345C mutant domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 0.167
-
 reduced acetylpyridine adenine dinucleotide reverse reaction of R350C mutant domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 0.283
-
 reduced acetylpyridine adenine dinucleotide reverse reaction of K424C mutant domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 0.283
-
 reduced acetylpyridine adenine dinucleotide reverse reaction of R425C mutant domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 0.55
-
 reduced acetylpyridine adenine dinucleotide reverse reaction of A348C mutant domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 0.567
-
 reduced acetylpyridine adenine dinucleotide reverse reaction of G430C mutant domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 0.917
-
 reduced acetylpyridine adenine dinucleotide reverse reaction of D392C mutant domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 10
-
 reduced acetylpyridine adenine dinucleotide cyclic reaction of R425C mutant domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 11.7
-
 reduced acetylpyridine adenine dinucleotide cyclic reaction of G430C mutant domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 15
-
 reduced acetylpyridine adenine dinucleotide cyclic reaction of D392C mutant domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 20.8
-
 reduced acetylpyridine adenine dinucleotide cyclic reaction of H345C mutant domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 33.3
-
 reduced acetylpyridine adenine dinucleotide cyclic reaction of R350C mutant domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 36.7
-
 reduced acetylpyridine adenine dinucleotide cyclic reaction of K424C mutant domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 40
-
 reduced acetylpyridine adenine dinucleotide cyclic reaction of D393C mutant domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 51.7
-
 reduced acetylpyridine adenine dinucleotide cyclic reaction of A432C mutant domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 55
-
 reduced acetylpyridine adenine dinucleotide cyclic reaction of A348C mutant domain III/R. rubrum domain I mixture Escherichia coli
7.1.1.1 81.7
-
 reduced acetylpyridine adenine dinucleotide cyclic reaction of wild-type domain III/R. rubrum domain I mixture Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.1.3 NADPH
-
 Escherichia coli
7.1.1.1 NAD+
-
 Escherichia coli
7.1.1.1 NAD+
-
 Rhodospirillum rubrum
7.1.1.1 NADH
-
 Escherichia coli
7.1.1.1 NADH
-
 Rhodospirillum rubrum
7.1.1.1 NADP+
-
 Escherichia coli
7.1.1.1 NADP+
-
 Rhodospirillum rubrum
7.1.1.1 NADPH
-
 Escherichia coli
7.1.1.1 NADPH
-
 Rhodospirillum rubrum