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Literature summary extracted from

  • French, C.E.; Boonstra, B.; Bufton, K.A.; Bruce, N.C.
    Cloning, sequence, and properties of the soluble pyridine nucleotide transhydrogenase of Pseudomonas fluorescens (1997), J. Bacteriol., 179, 2761-2765.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.1.1 expression in Escherichia coli Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.1.1 54000
-
 x * 54000, SDS-PAGE Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.6.1.1 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.1.1 affinity chromatography, native and recombinant enzyme Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
1.6.1.1 -20°C, 50 mM Tris-HCl, pH 7.0, 2 mM dithiothreitol, several weeks, no loss of activity, great loss of activity after several months Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.1.1 NADP+ + NADH
-
 Pseudomonas fluorescens NADPH + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.1.1 octamer x * 54000, SDS-PAGE Pseudomonas fluorescens